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Antibodies

Histone H3T11ph antibody (pAb)

Catalog No: 39151 Format: 200 µl $420 Buy Now
Catalog No: 39152 Format: 10 µl $95 Buy Now

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Antibody Type:Polyclonal
Isotype:Serum
Purification:None
Host:Rabbit
Molecular Weight:17 kDa
Reactivity:Human, Other (Wide Range)

Applications

ChIP Validated ChIP-Seq Validated Western Blot Validated Dot Blot Validated

for Histone H3 phospho Thr11 antibody (pAb)Application Notes


Validated Applications:
ChIP: 5 µl per ChIP
ChIP-Seq: 5 µl each
WB: 1:500 - 1:2,000 dilution

Published Applications:

WB

for Histone H3 phospho Thr11 antibody (pAb)Immunogen

This Histone H3 phospho Thr11 antibody was raised against a peptide including phospho-threonine 11 of histone H3.

for Histone H3 phospho Thr11 antibody (pAb)Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. For your convenience, an IgG version (Catalog No. 61197) of this antibody that was purified by Protein A Chromatography is also available.

for Histone H3 phospho Thr11 antibody (pAb)References

 
Histone H3T11ph antibody (pAb) tested by ChIP-Seq.

Histone H3 phospho Thr11 pAb tested by ChIP-Seq.
ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with chromatin from the human H9 embryonic stem cell line (4.5 million cells) and 5 ul of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 22 million sequence tags were mapped to identify H3T11ph occupancy. The image on the left shows H3T11ph binding at transcriptional start sites across a 120,000 bp region on chromosome 1. The image on the right is zoomed in to show H3T11ph binding at a single promoter

Histone H3T11ph antibody (pAb) tested by Western blot.

.Histone H3 phospho Thr11 pAb tested by Western blot.
Western blot probed with Histone H3 phospho Thr11 pAb (1:2,000 dilution).
    Lane 1: 200 ng recombinant histone H3.
    Lane 2: 5 µg acid extract of HeLa cells.
    Lane 3: 5 µg acid extract of HeLa cells treated with colcemid.

Histone H3T11ph antibody (pAb) tested by dot blot analysis.

Histone H3 phospho Thr11 pAb tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H3 phospho Thr11 pAb for phospho-Thr11 of histone H3. Decreasing amounts of peptides corresponding to regions around major sites of histone H3 threonine phosphorylation were spotted onto PVDF and probed with the antibody at a dilution of 1:10,000.
    Lane 1: Peptide phosphorylated at threonine 3.
    Lane 2: Unmodified threonine 3 peptide.
    Lane 3: Peptide phosphorylated at threonine 11.
    Lane 4: Unmodified threonine 11 peptide.
    Lane 5: Peptide phosphorylated at threonine 45.
    Lane 6: Unmodified threonine 45 peptide.

for Histone H3 phospho Thr11 antibody (pAb)Background

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.

Phosphorylation of several residues of histone H3, such as Ser10 (Histone H3 phospho Ser10), Ser28 (Histone H3 phospho Ser28) and Thr11, is tightly correlated with chromosome condensation during both mitosis and meiosis. Localization of histone H3 phospho Thr11 is different in mammals and in plants. In plant cells, phosphorylation of Thr11 of histone H3 is distributed along the entire length of condensed chromosome. In mammals, this modification is restricted to the centromeric region of the chromosome. Thr11 of histone H3 is phosphorylated by Dlk/ZIP kinases during mitosis.

for Histone H3 phospho Thr11 antibody (pAb)Positive Control

Active Motif's HeLa acid extract (Paclitaxel treated) (Catalog No. 36201) can be used as a positive control for Histone H3 phospho Thr11 antibody.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Histone H3T11ph antibody (pAb).

for Histone H3 phospho Thr11 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Histone H3 phospho Thr11 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

Histone H3T11ph antibody (pAb)
 

Data Thumbnails

Histone H3T11ph antibody (pAb) tested by ChIP-Seq.

ChIP-Seq of Histone H3T11ph pAb.
(Click image to enlarge and see details.)

Histone H3T11ph antibody (pAb) tested by Western blot.

Western blot of Histone H3T11ph pAb.
(Click image to enlarge and see details.)

Histone H3T11ph antibody (pAb) tested by dot blot analysis.

Dot blot of Histone H3T11ph pAb.
(Click image to enlarge and see details.)