Nucleophosmin antibody (pAb)
|Aliases: NPM1, B23, NPM|
|Catalog No: 39849||Format: 100 µl||$370||Buy Now|
|Catalog No: 39850||Format: 10 µl||$80||Buy Now|
for Nucleophosmin antibody (pAb)Application Notes
This antibody has been validated for use in:
WB: 1:500 - 1:2,000 dilution
for Nucleophosmin antibody (pAb)Immunogen
This Nucleophosmin antibody was raised against a peptide within the C-terminal region of human nucleophosmin.
for Nucleophosmin antibody (pAb)Buffer
Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
for Nucleophosmin antibody (pAb)Background
Nucleophosmin (NPM, NPM1, Nucleolar phosphoprotein B23) is a highly phosphorylated nucleolar protein involved in ribosome biogenesis, maintenance of genome stability, is directly implicated in cancer pathogenesis and serves as a histone chaperone. Nucleophosmin is closely related to Nucleoplasmin and shuttles back and forth between the nucleolus and the nucleoplasm. Chromosomal aberrations involving Nucleophosmin were found in patients with non-Hodgkin lymphoma, acute promyelocytic leukemia, myelodysplastic syndrome, and acute myelogenous leukemia. Nucleophosmin has been shown to interact with Nucleolin, AKT1, BRCA1 and BARD1. Mice deficient for Nucleophosmin have severe defects in genome stability due to unrestricted centrosome duplication. Heterozygous Nucleophosmin mutant mice show signs of myelodysplastic syndrome.
for Nucleophosmin antibody (pAb)Positive Control
Active Motif's Jurkat nuclear extract (Catalog No. 36014) can be used as a positive control for Nucleophosmin antibody.
for Nucleophosmin antibody (pAb)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for Nucleophosmin antibody (pAb)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
- WB = Western Blotting