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Tools to analyze nuclear function,
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Antibodies

Nucleophosmin antibody (pAb)

Aliases: NPM1, B23, NPM
Catalog No: 39849 Format: 100 µl $370 Buy Now
Catalog No: 39850 Format: 10 µl $80 Buy Now
Antibody Type:Polyclonal
Isotype:Serum
Purification:None
Host:Rabbit
Molecular Weight:38 kDa
Reactivity:Human

Applications

Western Blot Validated

for Nucleophosmin antibody (pAb)Application Notes

This antibody has been validated for use in:
WB: 1:500 - 1:2,000 dilution

for Nucleophosmin antibody (pAb)Immunogen

This Nucleophosmin antibody was raised against a peptide within the C-terminal region of human nucleophosmin.

for Nucleophosmin antibody (pAb)Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

 
Nucleophosmin antibody (pAb) tested by Western blot.

Nucleophosmin antibody (pAb) tested by Western blot.
Nuclear extract of Jurkat cells (30 µg per lane) probed with Nucleophosmin antibody (pAb) at a dilution of 1:1,000.

for Nucleophosmin antibody (pAb)Background

Nucleophosmin (NPM, NPM1, Nucleolar phosphoprotein B23) is a highly phosphorylated nucleolar protein involved in ribosome biogenesis, maintenance of genome stability, is directly implicated in cancer pathogenesis and serves as a histone chaperone. Nucleophosmin is closely related to Nucleoplasmin and shuttles back and forth between the nucleolus and the nucleoplasm. Chromosomal aberrations involving Nucleophosmin were found in patients with non-Hodgkin lymphoma, acute promyelocytic leukemia, myelodysplastic syndrome, and acute myelogenous leukemia. Nucleophosmin has been shown to interact with Nucleolin, AKT1, BRCA1 and BARD1. Mice deficient for Nucleophosmin have severe defects in genome stability due to unrestricted centrosome duplication. Heterozygous Nucleophosmin mutant mice show signs of myelodysplastic syndrome.

for Nucleophosmin antibody (pAb)Positive Control

Active Motif's Jurkat nuclear extract (Catalog No. 36014) can be used as a positive control for Nucleophosmin antibody.

for Nucleophosmin antibody (pAb)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Nucleophosmin antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FACS = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

Nucleophosmin antibody (pAb)
 

Data Thumbnails

Nucleophosmin antibody (pAb) tested by Western blot.

Western blot of Nucleophosmin pAb.
(Click image to enlarge and see details.)