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GATA-4 antibody (pAb)
| RRID: AB_2615046 |
| Aliases: ASD2, VSD1, TACHD |
| Catalog No: 39893 | Format: 100 µl | $500 | Buy |
| Catalog No: 39894 | Format: 10 µl | $125 | Buy |
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Applications
Application Notes
Applications Validated by Active Motif:
WB: 1:500 - 1:2,000 dilution
Immunogen
This GATA-4 antibody was raised against a peptide derived from the C-terminus of human GATA-4.
Buffer
Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
GATA-4 antibody (pAb) tested by Western blot.
Western Blot: Nuclear extract (20 µg) of F9 cells (retinoic acid treated mouse teratocarcinoma F9 stem cell line, Active Motif Catalog no. 36113) probed with GATA-4 antibody at a dilution of 1:2,000.
Background
GATA-4 is a zinc finger protein and part of the GATA family of sequence specific DNA binding transcription factors that recognize a consensus DNA sequence containing GATA. GATA-4 is involved in the development of several mesodermal and endodermal structures. GATA-4 mutant mice exhibit severe heart malformations. Defects in GATA-4 are the cause of atrial septal defect type 2. ASD2 is a congenital heart malformation characterized by incomplete closure of the wall between the atria resulting in blood flow from the left to the right atria. GATA-4 also plays a role in regulating gene expression in cardiac and smooth muscle cell types.
Positive Control
Active Motif's F9 nuclear extract (Retinoic acid treated) (Catalog No. 36113) can be used as a positive control for GATA-4 antibody.
Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
Guarantee
This product is guaranteed for 12 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- WB = Western Blotting
