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Histone H2BK15ac antibody (pAb)
| RRID: AB_2793590 |
| Catalog No: 61321 | Format: 100 µl | $515 | Add to Cart |
| Catalog No: 61322 | Format: 10 µl | $130 | Add to Cart |
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Applications
Contents
A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and antibody concentration.
Immunogen
This antibody was raised against a peptide including acetyl-lysine 15 of human histone H2B.
Buffer
Purified IgG in PBS with 30% glycerol and 0.035% sodium azide.
View our Guide to Histone Modifications and Biological Function.
Histone H2B acetyl Lys15 pAb tested by Western blot.
Nuclear extract of HeLa cells (20 µg per lane) was probed with Histone H2B acetyl Lys15 polyclonal antibody (1:500 dilution).
Lane 1: Untreated cells.
Lane 2: Cells treated with sodium butyrate.
Histone H2B acetyl Lys15 pAb tested by dot blot analysis.
Specificity Data: Dot blot analysis was used to confirm the specificity of Histone H2B acetyl Lys15 pAb for acetyl-Lys 15 of histone H2B. Decreasing amounts of modified and unmodified peptides were spotted onto PVDF and probed with the antibody at a dilution of 1:750.
Lane 1: Peptide acetylated at lysine 5 of H2B.
Lane 2: Peptide acetylated at lysine 12 of H2B.
Lane 3: Peptide acetylated at lysine 15 of H2B.
Lane 4: Unmodified lysine 15 peptide.
Lane 5: Peptide acetylated at lysine 16 of H2B.
Lane 6: Peptide acetylated at lysine 46 of H2B.
Lane 7: Peptide acetylated at lysine 120 of H2B.
Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
Guarantee
This product is guaranteed for 12 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- TIP-ChIP = Tagmented, Indexed, and Pooled Chromatin Immunoprecipitation;
- WB = Western Blotting
