XRCC1 antibody (pAb)
RRID: AB_2793610 |
Aliases: RCC |
Catalog No: 61371 | Format: 100 µl | $500 | Buy |
Catalog No: 61372 | Format: 10 µl | $125 | Buy |
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Applications
Application Notes
Applications Validated by Active Motif:
ICC/IF: 1:2,000 dilution
WB*: 1:500 - 1:1,000 dilution
The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.
*Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.
Immunogen
This antibody was raised against a recombinant protein corresponding to the N-terminal region of human XRCC1.
Buffer
Purified IgG in PBS with 30% glycerol and 0.035% sodium azide.
Background
XRCC1 (X-ray repair complementing defective repair in Chinese hamster cells 1) corrects defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents. XRCC1 is involved in the efficient repair of DNA single-strand breaks formed by exposure to ionizing radiation and alkylating agents. This protein interacts with DNA ligase III, polymerase beta and poly (ADP-ribose) polymerase (PARP) to participate in the base excision repair (BER) pathway.
Positive Control
Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for XRCC1 antibody.
Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
Guarantee
This product is guaranteed for 12 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- WB = Western Blotting