| Antibody Type: | Polyclonal |
| Isotype: | Rabbit Serum |
| Purification: | None |
|
| Appl.: | IF, WB |
| Reactivity: | Human, Mouse, Other (Wide Range predicted) |
| Molecular Weight: | 49 kDa |
|
| Western Blot |
Chromatin IP |
Immunofluorescence |
| 1:500 - 1:5,000 |
Not tested |
1:200 - 1:1,000 |
Background for Aurora B antibody (pAb):
Aurora B (
Aurora Kinase B,
AurB,
ARK2) is a Ser/Thr kinase that is part of the chromosomal passenger complex that associates with chromosomes and is an important regulator of mitosis and meiosis.
Aurora B becomes concentrated at the centromere during the onset of mitosis, but relocates to the spindle midzone during anaphase.
Aurora B phosphorylates a number of substrates, including
INCENP, CENP-A, and
Histone H3. Misregulation of
Aurora B activity can lead to chromosome instability and is implicated in a number of cancers.
Immunogen for Aurora B antibody (pAb):
This antibody was raised against a peptide including the C-terminus of human Aurora B.
Application Notes for Aurora B antibody (pAb):
This Aurora B antibody has been validated for use in Western blotting (1:500 - 1:5,000 dilution) and immunofluorescence (1:200 - 1:1,000 dilution).
Buffer:
Rabbit serum containing 30% glycerol and 0.035% sodium azide.
Positive Control:
Active Motif's
HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for Aurora B antibody.
Compatible Secondary Antibodies:
Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for your experiments.
Aurora B pAb tested by immunofluorescence.
Top left: HeLa cells stained with Aurora B polyclonal antibody at a 1:200 dilution. Top right: Same cells stained with alpha Tubulin mAb. Bottom left: Same cells stained with DAPI. Bottom right: Merge of all 3 images.
Aurora B pAb tested by Western blot
Nuclear extract of U2OS cells (20 µg per lane) probed with Aurora B polyclonal antibody (1:500 dilution).
Storage:
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Guarantee:
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key:
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- IF = Immunofluorescence;
- ICC = Immunocytochemistry;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
YOUR CART
