| Antibody Type: | Monoclonal |
| Isotype: | Mouse IgG1 |
| Purification: | Affinity Purified |
|
| Appl.: | WB |
| Reactivity: | Human, Mouse, Rat |
| Molecular Weight: | 35, 12, and 17 kDa |
|
| Western Blot |
Chromatin IP |
| 2 - 4 µg/ml |
Not tested |
Background for Caspase-3 antibody (mAb):
Caspase-3 (
CASP3 or
apoptosis-related cysteine peptidase 3) is involved in the activation cascade of caspases responsible for apoptosis execution.
Caspase-3 cleaves and activates Caspase-6,
Caspase-7 and
Caspase-9, and the protein itself is processed by
Caspase-8,
Caspase-9 and Caspase-10.
Caspase-3 is the predominate caspase involved in the cleavage of amyloid-beta 4A, which is associated with Alzheimer's disease.
Immunogen for Caspase-3 antibody (mAb):
This antibody was raised against a 6xHis-tagged, full-length human Caspase-3 protein.
Application Notes for Caspase-3 antibody (mAb):
This Caspase-3 antibody has been validated for use in Western blotting (2 - 4 µg/ml dilution). For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.
Buffer:
PBS containing 0.02% sodium azide. Sodium azide is highly toxic.
Positive Control:
Active Motif's
HeLa whole-cell extract (Catalog No. 40050) can be used as a positive control for Caspase-3 antibody.
Compatible Secondary Antibodies:
Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for your experiments.
Caspase-3 mAb tested by Western blot.
Western blot analysis for detection of Caspase-3 activation. HeLa cells were treated with 2 µM staurosporine for different time periods and then probed using Caspase-3 mAb. Caspase-3 activation is determined by cleavage of pro-Caspase-3, which generates 17 and 12 kDa proteins, the larger and smaller catalytic subunit, respectively.
Storage:
Antibodies in solution can be stored at -80°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Guarantee:
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key:
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- IF = Immunofluorescence;
- ICC = Immunocytochemistry;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
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