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Recombinant Proteins & Enzymes

Recombinant HDAC9 protein, active

Catalog No: 31354 Format: 10 µg $345 Buy Now

Expressed In:

Sf9 cells

Protein Species:

Human

for Recombinant HDAC9 protein, activeContents

5 µg recombinant HDAC9 expressed in Sf9 cells supplied in a buffer of 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 250 mM imidazole, 10% glycerol.

for Recombinant HDAC9 protein, activeApplication Notes

Recombinant HDAC9 is suitable for use in histone deacetylase (HDAC) assays. A good starting point is 5 to 25 ng of enzyme per assay.

for Recombinant HDAC9 protein, activeProtein Details

HDAC9 is a Class II histone deacetylase with broad specificity. The C-terminal part of HDAC9 (accession number NP_848510) including the catalytic domain (amino acids 604-1066) was expressed with an C-terminal His tag (MW= 51 kDa) in Sf9 cells using a baculovirus expression system.

 
HDAC9 activity assay

HDAC9 activity assay.
Recombinant HDAC9 activity measured using a fluorescent HDAC assay.

HDAC9 Coomassie gel

HDAC9 protein gel.
HDAC9 (5 μg) run on an SDS-PAGE gel and stained with Coomassie blue.

for Recombinant HDAC9 protein, activeStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant HDAC9 protein, activeGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Data Thumbnails

HDAC9 activity assay

HDAC9 activity assay.
(Click image to enlarge and see details).

HDAC9 Coomassie gel

HDAC9 Coomassie stained gel.
(Click image to enlarge and see details).