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for Recombinant HDAC9 protein, activeContents
5 µg recombinant HDAC9 expressed in Sf9 cells supplied in a buffer of 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 250 mM imidazole, 10% glycerol.
for Recombinant HDAC9 protein, activeApplication Notes
Recombinant HDAC9 is suitable for use in histone deacetylase (HDAC) assays. A good starting point is 5 to 25 ng of enzyme per assay.
for Recombinant HDAC9 protein, activeProtein Details
HDAC9 is a Class II histone deacetylase with broad specificity. The C-terminal part of HDAC9 (accession number NP_848510) including the catalytic domain (amino acids 604-1066) was expressed with an C-terminal His tag (MW= 51 kDa) in Sf9 cells using a baculovirus expression system.
HDAC9 activity assay.
Recombinant HDAC9 activity measured using a fluorescent HDAC assay.
HDAC9 protein gel.
HDAC9 (5 μg) run on an SDS-PAGE gel and stained with Coomassie blue.
for Recombinant HDAC9 protein, activeStorage
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for Recombinant HDAC9 protein, activeGuarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.


