dna-binding ELISA for activated estrogen receptor
TransAM® Kits are DNA-binding ELISAs that facilitate the study of transcription factor activation in mammalian tissue and cell extracts. Assays are available for over 40 different targets (see the list at right). Each kit includes a 96-stripwell plate in which multiple copies of a specific double-stranded oligonucleotide have been immobilized. When nuclear or whole-cell extract is added, activated transcription factor of interest binds the oligonucleotide at its consensus binding site and is quantified using the included antibody, which is specific for the bound, active form of the transcription factor being studied. For complete details, click the TransAM® Method tab below.
TransAM® ER Transcription Factor ELISA Kits
TransAM ER Kits provide everything needed to study activated Estrogen Receptor alpha (ERα), including a positive control extract. The ER kit detects ERα from human, mouse and rat extracts. Click the ER Info tab below for data and more information; kit manuals can be downloaded under the Documents tab.
|TransAM® ER||1 x 96 rxns||41396||$665||Buy Now|
|5 x 96 rxns||41996||$2,795||Buy Now|
|TransAM® ER Manual|
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Estrogen receptors (ER) are nuclear hormone receptors that mediate the actions of the endogenous steroid hormone, 17β-estradiol (E2). ER is synthesized as two protein forms, ERα and ERβ, which can bind to the estrogen receptor element (ERE) as homo- or heterodimers. Cell proliferation and differentiation triggered by ER activation has been heavily studied in bone-wasting and breast cancer treatments. Estrogen receptors are necessary for sexual development and reproductive function. The TransAM ER Kit contains an antibody specific for the active form of ERα (also known as estrogen receptor 1 or ESR1) when it is bound to the target DNA.
Figure 1: Monitoring ERα activation with the TransAM ER Kit.
The TransAM® transcription factor ELISA advantage
Historically, transcription factor studies have been conducted using gelshift, Western blot and reporter plasmid transfections, which are time-consuming, do not allow for high-throughput and provide only semi-quantitative results. TransAM assays are up to 100 times more sensitive than gelshift techniques, and can be completed in less than 5 hours. Because TransAM is an ELISA-based assay*, there is no radioactivity, and the high-throughput stripwell format enables simultaneous screening of 1-96 samples. Inconsistencies due to variable reporter plasmid transfections are eliminated, along with the need to construct stable cell lines.
Why use TransAM® transcription factor ELISAs?
- Up to 100-fold more sensitive than gelshift assays
- Eliminates the use of radioactivity and the need to run gels
- Results in less than five hours
- Colorimetric readout enables easy, quantitative analysis with spectrophotometry at 450 nm
- 96-stripwell format enables both high and low throughput
How TransAM® transcription factor ELISAs work
The TransAM format is perfect for assaying transcription factor binding to a consensus-binding site. TransAM Kits contain a 96-stripwell plate to which the consensus-binding site oligo has been immobilized. Activated nuclear extract is added to each well and the transcription factor of interest binds specifically to this bound oligonucleotide. A primary antibody specific for an epitope on the bound and active form of the transcription factor is then added followed by subsequent incubation with secondary antibody and Developing Solution to provide an easily quantified, sensitive colorimetric readout (Figure 1).
Figure 1: Flow chart of the TransAM process.
Contents & Storage
One or five ER 96-well plate(s) with plate sealer(s), ERα primary antibody, HRP-conjugated secondary antibody, ER wild-type and mutated oligonucleotides, positive control cell extract, Dithiothreitol, Protease Inhibitor Cocktail, Lysis, Binding, 10X Washing and 10X Antibody Binding Buffers, Developing and Stop Solutions and Poly[d(I-C)]. Reagent storage conditions vary from room temperature to -80°C, see manual for details. All reagents are guaranteed stable for 6 months.