Description

ChIP-IT Express HT offers high throughput chromatin IP

ChIP-IT Express HT combines the efficiency and time-savings of the magnetic bead-based protocol of ChIP-IT Express with the ability to process up to 96 ChIP reactions simultaneously. The ChIP-IT Express HT Kit contains components to perform 96 ChIP reactions. The included LSV (Low Sample Volume) Protein G-coated magnetic beads are provided ready to use. These beads have a high binding capacity for IgG, enabling them to be used in smaller volumes that reduce non-specific binding. Because of this lower non-specific binding, magnetic beads require fewer washing steps than agarose beads, and it is not necessary to pre-clear the chromatin.

An added advantage is that the magnetic beads pellet much more quickly than agarose beads, which are pelleted by centrifugation. In addition, magnetic stands are designed to pellet the beads onto the side of the tube. This makes it easy to remove buffers without disturbing the beads, so washing can be performed using multi-channel pipettors. This dramatically reduces hands-on time and ensures sample-to-sample consistency. The LSV Protein-G coated magnetic beads are also optimized for use in the small sample size of a microplate well.

For a limited time, you will receive a free MAG-96 magnetic stand when you purchase ChIP-IT Express HT (while quantities last).

Figure 1: High-throughput ChIP with ChIP-IT Express HT.

Now you can perform 96 ChIP reactions simultaneously with ChIP-IT Express HT and a multi-channel pipettor. The magnetic bead-based protocol enables faster washing steps.

Figure 2: Use of the MAG-96 plate sorter with ChIP-IT Express HT.

ChIP-IT Express HT can be used with Active Motif's MAG-96 magnetic sorter or any commercially available 96-well magnetic sorter for rapid sorting and washing of the magentic beads in ChIP-IT Express HT.

Figure 3: MAG-96 magnetic sorter stand.

Ideal for sorting and washing the magnetic beads in the ChIP-IT Express HT Kit.

Choose from sonication or reproducible enzymatic shearing

The first step in successful ChIP is shearing the chromatin into 200-1000 bp fragments. This has traditionally been performed using sonication. Although sonication is effective, many users find that complications caused by emulsification and overheating can lead to variable results. To improve reproducibility, Active Motif has developed a unique enzymatic shearing method. The ChIP-IT Express HT Kit is compatible with sonication or enzymatic shearing formats. Click here to learn more about your options. And, to make you ChIP even simpler, we also offer Ready-to-ChIP Chromatin.