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LightSwitch™ 3´UTR Control Vectors

3´UTR controls help optimize luciferase assay conditions

Because no single control can be ideal for all experimental conditions, Active Motif offers a panel of positive and negative control constructs for the LightSwitch™ 3´UTR Reporter Vector. These controls are used to measure background, to optimize experimental conditions and to normalize for non-specific effects associated with a treatment or change in condition. Note that the unique RenSP luciferase technology utilized in the LightSwitch Assay System eliminates the need for co-transfection of a normalizing control in most cases. So, instead of one control construct being co-transfected with every experimental plasmid, different control vectors can be used in your reporter assay experiment, each in its own well, in addition to the constructs being tested. Using the most suitable positive and negative control vectors for your specific experiment will give you the highest degree of confidence in your results.

Information on all LightSwitch 3´UTR Control Vectors is shown under the 3´UTR Controls tab below.

IMPORTANT:  Because all LightSwitch reporter constructs contain the optimized RenSP luciferase gene, you MUST use our LightSwitch Luciferase Assay Reagents to obtain optimal results. (Other luciferase assay reagents are not compatible with RenSP.)

 
Name Type of Control Product Info Format Cat No. Price  
LightSwitch™ ACTB 3´UTR Control Positive control Get Info 5 µg 32013 $250 Buy Now
LightSwitch™ GAPDH 3´UTR Control Positive control Get Info 5 µg 32014 $250 Buy Now
LightSwitch™ LDHA 3´UTR Control Positive control Get Info 5 µg 32015 $250 Buy Now
LightSwitch™ PPIA 3´UTR Control Positive control Get Info 5 µg 32016 $250 Buy Now
LightSwitch™ Random 3´UTR Control 1 Negative control Get Info 5 µg 32017 $250 Buy Now
LightSwitch™ Random 3´UTR Control 2 Negative control Get Info 5 µg 32018 $250 Buy Now
LightSwitch™ Random 3´UTR Control 3 Negative control Get Info 5 µg 32019 $250 Buy Now
LightSwitch™ Random 3´UTR Control 4 Negative control Get Info 5 µg 32020 $250 Buy Now
LightSwitch™ 3´UTR Reporter Vector Empty vector Vector Info 5 µg 32022 $250 Buy Now

Empty 3´UTR vector: The empty 3´UTR vector contains the RenSP luciferase reporter gene and its constitutive promoter. This construct may serve as a positive control for the transfection because of its promoter. It may also be utilized for creating additional constructs. For a vector map, annotations and primer & restriction sites, please see the Empty LightSwitch™ Reporter Vectors page.

Housekeeping gene 3´UTR vectors: Housekeeping control constructs contain the 3´UTRs for common housekeeping genes cloned downstream of the RenSP luciferase reporter gene.

Random control 3´UTR vectors: Random control constructs contain non-conserved, non-genic, and non-repetitive human genomic fragments.

pLightSwitch_3UTR vector diagram
Diagram depicting the luciferase activity of the positive and negative LightSwitch 3´UTR Controls
Figure 1: LightSwitch assay performed using the positive and negative LightSwitch 3´UTR Controls.

Fifty ng of each 3´UTR Control Vector was transfected into HT1080 cells. Because the constitutive promoter driving the RenSP luciferase gene is the same for all vectors, it is expected that all of the vectors will give comparable signals, as this experiment does not include miRNAs to interact with the various 3´UTR regions and change the amount of luciferase produced.
The Y-axis values are shown in log scale. Absolute and relative activity will vary depending on cell line, experimental conditions and transfection reagents.

3´UTRs of housekeeping genes

Catalog No. Name Description Coordinates Sequence
32013 LightSwitch™ ACTB
3´UTR Control
Beta-actin chr7–:
5340002 - 5340681
Get Info
32014 LightSwitch™ GAPDH
3´UTR Control
Glyceraldehyde-3-phosphate
dehydrogenase
chr12+:
6517534 - 6517833
Get Info
32015 LightSwitch™ LDHA
3´UTR Control
Lactose dehydrogenase A chr11+:
18385382 - 18386064
Get Info
32016 LightSwitch™ PPIA
3´UTR Control
Peptidylprolyl isomerase A chr7+:
44614226 - 44616012
Get Info

Random genomic fragments in 3´UTR vector

Catalog No. Name Coordinates Sequence
32017 LightSwitch™ Random
3´UTR Control 1
chr13+:
82190847 - 82193385
Get Info
32018 LightSwitch™ Random
3´UTR Control 2
chr12+:
82120892 - 82121673
Get Info
32019 LightSwitch™ Random
3´UTR Control 3
chr13+:
26144830 - 26145608
Get Info
32020 LightSwitch™ Random
3´UTR Control 4
chr22+:
1645930 - 1646850
Get Info