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for Recombinant GSK3b protein, activeContents
10 µg of Recombinant GSK3β protein in 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20% glycerol. Protein is supplied at 0.276 µg/µl.
for Recombinant GSK3b protein, activeApplication Notes
Recombinant GSK3β is suitable for kinase assays and Western blot. The molecular weight of the protein is ~76.312 kDa. The activity of the protein is ~ 2 pmol/µg min. Recommended kinase reaction conditions: 60 mM HEPES-NaOH, pH 7.5, 3 mM MgCl2, 3 mM MnCl2, 3 µM Na-orthovanadate, 1.2 mM DTT, ATP (variable), 2.5 µg/50 µl PEG20.000, Substrate: Rb-CTF 5 µg/50 µl, Recombinant GSK3β: 200 ng/50 µl. Kinase activity may vary depending on the substrate and reaction conditions used.
for Recombinant GSK3b protein, activeProtein Details
Recombinant human GSK3β protein was produced using baculovirus infected Sf9 cells. The protein was made against amino acids M1-T420 , accession number L33801 and N-terminally fused to GST-HIS6-Thrombin cleavage site. Purified by GSH-agarose affinity purification.
Recombinant GSK3β protein tested by SDS-PAGE gel.
0.5 µg of GSK3β protein was analyzed using SDS/PAGE followed by Western blot with either an anti-GSK3β or anti-GST antibody.
for Recombinant GSK3b protein, activeGuarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
