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for Recombinant IKKe protein, activeContents
10 µg of Recombinant IKKε protein in 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol. Protein is supplied at 0.069 µg/µl.
for Recombinant IKKe protein, activeApplication Notes
Recombinant IKKε is suitable for kinase assays and Western blot. The molecular weight of the protein is ~110.059 kDa. The activity of the protein is ~ 8 pmol/µg min. Recommended kinase reaction conditions: 60 mM HEPES-NaOH, pH 7.5, 3 mM MgCl2, 3 mM MnCl2, 3 µM Na-orthovanadate, 1.2 mM DTT, ATP (variable), 2.5 µg/50 µl PEG20.000, Substrate: Casein 10 µg/50 µl, Recombinant IKKε : 100 ng/50 µl. Kinase activity may vary depending on the substrate and reaction conditions used.
for Recombinant IKKe protein, activeProtein Details
Recombinant human IKKε protein was produced using baculovirus infected Sf9 cells. The protein was made against amino acids M1-V716, accession number NM_014002 and N-terminally fused to GST-HIS6-Thrombin cleavage site. Purified by GSH-agarose affinity purification.
for Recombinant IKKe protein, activeGuarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
