Recombinant Histone H2B

for Recombinant Histone H2BContents

50 µg supplied as lyophilized powder.

for Recombinant Histone H2BBackground

Histone H2B is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of H2A, H2B, H3 and H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points.

for Recombinant Histone H2BApplication Notes

Recombinant histones are suitable for use as substrates for in vitro enzymatic reactions and chromatin assembly. Each lot has been evaluated by SDS-PAGE and dot blot analysis.

for Recombinant Histone H2BProtein Details

Recombinant Xenopus laevis Histone H2B produced in E. coli and purified using FPLC. The H2B protein is missing the first three residues (PEP). Protein concentration was determined by spectrophotometry. ≥98% purity by SDS-PAGE. The MW of the protein is 13,494 Daltons. Resuspend protein in suitable buffer for your assay. The protein sequence is as follows: AKSAPA PKKGSKKAVT KTQKKDGKKR RKSRKESYAI YVYKVLKQVH PDTGISSKAM SIMNSFVNDV FERIAGEASR LAHYNKRSTI TSREIQTAVR LLLPGELAKH AVSEGTKAVT KYTSAK

for Recombinant Histone H2BStorage

Lyophilized proteins can be stored at -20°C or -80°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant Histone H2BGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.