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for Recombinant SIRT6 protein, activeContents
25 µg recombinant SIRT6 protein supplied at a concentration of 0.2 µg/µl in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20 and 20% glycerol.
for Recombinant SIRT6 protein, activeApplication Notes
Recombinant SIRT6 is suitable for use in histone deacetylase assays. A recommended starting point for the HDAC assay is 20-100 ng of recombinant protein per reaction.
for Recombinant SIRT6 protein, activeProtein Details
SIRT6 is a histone and protein deacetylase, an NAD-dependant enzyme that removes acetyl groups from lysine acetylated proteins. SIRT6 has been shown to deacetylate histone H3 at lysine 9. Recombinant human SIRT6 was expressed in E. coli as the full length protein (accession number NM_016539) with an N-terminal GST tag. The molecular weight of the protein is ~65 kDa.
SIRT6 protein gel.
HDAC1 (6 μg) run on a 10% SDS-PAGE gel and stained with Coomassie blue. Arrow indicates recombinant SIRT6.
for Recombinant SIRT6 protein, activeGuarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
