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Recombinant Proteins & Enzymes

Recombinant KMT2B (MLL2) complex

Catalog No: 31498 Format: 20 µg $415 Buy Now
Catalog No: 31902 Format: 1 mg $3,500 Buy Now
Expressed In: E. coli Protein Species: Human

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for Recombinant KMT2B (MLL2) complexContents

Recombinant KMT2B (MLL2) complex was expressed in E.coli cells and is supplied in 25 mM Tris pH 8.0, 300 mM NaCl, 5% Glycerol, 0.04% Triton X-100. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant KMT2B (MLL2) complexBackground

KMT2B (MLL2) complex (Myeloid/lymphoid or mixed-lineage leukemia 2) is a Trithorax-group protein that function collectively to promote gene expression. KMT2B (MLL2) is a histone methyltransferase that methylates the Lysine 4 position of histone H3. In particular, the SET domain is a conserved C-terminal domain that characterizes proteins of the MLL (mixed-lineage leukemia) family. The SET domain is responsible for its histone methyltransferase activity which mediates chromatin modifications associated with epigenetic transcriptional activation. H3K4 methylation represents a specific epigenetic tag for transcriptional activation. MLL2 is part of a large protein complex called ASCOM, which has been shown to be a transcriptional regulator of the β-globin and estrogen receptor genes. Mutations in this gene have been shown to be a cause of Kabuki syndrome.

for Recombinant KMT2B (MLL2) complexApplication Notes

Recombinant KMT2B (MLL2) complex is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.

HMT Assay Conditions: 3.3 μM H3K4me0 (1-21aa) peptide was incubated with different concentrations of recombinant MLL2 complex in reaction buffer containing 50 mM TrisCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP, 100 μM SAM for 3 hours at room temperature. Activity was detected by HTRF and MALDI-TOF.

for Recombinant KMT2B (MLL2) complexProtein Details

Recombinant KMT2B (MLL2) complex contains amino acids 5355 - 5537 of the human MLL2 protein (accession number NP_003473.3) with N-terminal GST-Tag and MW = 47.6 kDa; full length human WDR5 (GenBank Accession No.NM_017588), with N-terminal 6×His-Tag and MW = 35 kDa; full length human ASH2L (GenBank Accession No. NM_001105214), N-terminal 6×His-Tag and MW = 61 kDa; full length human RbBP5 (GenBank Accession No. NM_005057), N-terminal 6×His-Tag and MW = 60 kDa, and full length human DPY30 (GenBank Accession No. NM_032574), N-terminal 6×His-Tag and MW =12 kDa, all individually expressed in E. coli cells. The recombinant protein is >80% pure by SDS-PAGE.

 
KMT2B (MLL2) complex Coomassie gel

Recombinant KMT2B (MLL2) complex gel
MLL2 complex was run on an 10% SDS-PAGE gel and stained with Coomassie blue.

KMT2B (MLL2) complex HTRF activity assay

Recombinant KMT2B (MLL2) complex HTRF activity assay
3.3 μM H3K4me0 (1-21aa) peptide was incubated with MLL2 complex in reaction buffer for 3 hour at room temperature. MLL2 complex was used in a HTRF assay to determine enzyme linearity. Methylated peptide (H3K4me2) was measured using H3K4me2-specific antibody.

KMT2B (MLL2) complex MALDI-TOF activity assay

Recombinant KMT2B (MLL2) complex MALDI-TOF activity assay
3.3 uM H3K4me0 peptide was incubated with 10 nM MLL2 complex in reaction buffer for 3 hours at room temperature. The reaction product was detected by MALDI-TOF. Single 3.3 uM H3K4me0 peptide was used as negative control.

Catalytic Ability: ~450 turnovers/ enzyme molecule

for Recombinant KMT2B (MLL2) complexStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant KMT2B (MLL2) complexGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Recombinant KMT2B (MLL2) complex
 

Data Thumbnails

KMT2B (MLL2) complex Coomassie gel

KMT2B (MLL2) complex Coomassie gel.
(Click image to enlarge and see details).

KMT2B (MLL2) complex HTRF activity assay

KMT2B (MLL2) complex HTRF activity assay.
(Click image to enlarge and see details).

KMT2B (MLL2) complex MALDI-TOF activity assay

KMT2B (MLL2) complex MALDI-TOF activity assay.
(Click image to enlarge and see details).