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Recombinant Proteins & Enzymes

Recombinant MAOA protein

Aliases: Monoamine Oxidase A, MAO-A
Catalog No: 31502 Format: 10 µg $395 Buy Now
Expressed In: Baculovirus Protein Species: Human

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for Recombinant MAOA proteinContents

Full length recombinant MAOA protein was expressed in Sf9 cells and is supplied in 25 mM Hepes pH 7.5, 300 mM NaCl, 5% Glycerol, 0.04% Triton X-100, 0.2 mM TCEP, 0.2 mg/ml 3× Flag peptide. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant MAOA proteinBackground

MAOA (Monoamine Oxidase A) is one of two neighboring mitochondrial enzyme family members which catalyze the oxidative deamination of amines, such as dopamine, norepinephrine, and serotonin. The other member is MAOB. Mutation of MAOA results in Brunner syndrome and maoa-related behavior disorders. MAOA has important functions in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. MAOA preferentially oxidizes biogenic amines such as 5-hydroxytryptamine (5-HT), norepinephrine and epinephrine.

for Recombinant MAOA proteinApplication Notes

Recombinant MAOA is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.

Monoamine Oxidase Assay: 40 uM substrate was incubated with MAOA protein in reaction buffer for 1 hour at room temperature, then the product was incubated with Fluorescence for 0.5 hour at temperature. The activity of MAOA was detected by Promega MAO-GloTM Assay.

for Recombinant MAOA proteinProtein Details

Recombinant MAOA (accession number NP_000231.1) was expressed in Sf9 cells and contains an N-terminal FLAG-Tag with an observed molecular weight of 60.7 kDa. The recombinant protein is >80% pure by SDS-PAGE.

 
MAOA protein Coomassie gel

Recombinant MAOA protein gel.
MAOA protein was run on an 8% SDS-PAGE gel and stained with Coomassie blue.

MAOA protein activity assay

Recombinant MAOA activity assay.
40 uM substrate was incubated with MAOA protein in reaction buffer for 1 hour at room temperature, then the product was incubated with Fluorescence for 0.5 hour at temperature. The activity of MAOA was detected by Promega MAO-GloTM Assay.

for Recombinant MAOA proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant MAOA proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Recombinant MAOA protein
 

Data Thumbnails

MAOA protein Coomassie gel

MAOA protein Coomassie gel.
(Click image to enlarge and see details).

MAOA protein activity assay

MAOA protein activity assay.
(Click image to enlarge and see details).