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Recombinant Proteins & Enzymes

Recombinant IDH2 protein

Catalog No: 31615 Format: 100 µg $395 Buy Now
Expressed In: Baculovirus Protein Species: Human

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for Recombinant IDH2 proteinContents

Recombinant IDH2 protein is supplied at a concentration of 0.5 µg/µl in 25 mM HEPES pH 7.5, 300 mM NaCl, 5% glycerol, 0.04% Triton X-100, 0.2 mM TCEP.

for Recombinant IDH2 proteinBackground

IDH2 (Isocitrate Dehydrogenase (NADP(+)) 2, Mitochondrial, also known as MNADP-IDH, D2HGA2, IDHM, IDH, IDPM, IDC-M) is a member of isocitrate dehydrogenases, which catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. Isocitrate dehydrogenases belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer.
IDH2 is a NADP(+)-dependent isocitrate dehydrogenase found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex.

for Recombinant IDH2 proteinApplication Notes

This protein is useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Activity Assay Conditions: 200 µM DL-isocitric acid trisodium and 200 µM NADP+ were incubated with IDH2 protein in reaction buffer including 50 mM Tris-HCl pH 7.4, 100 mM MgCl2 for 10 minutes at room temperature. The generation of product NADPH was monitored by measuring absorbance at 340 nm.

for Recombinant IDH2 proteinProtein Details

Recombinant IDH2 protein was expressed in Baculovirus system as the full length protein (accession number NP_002159.2) with a C-terminal FLAG-Tag. The molecular weight of the protein is 51.7 kDa.

 
Recombinant IDH2 protein, SDS-PAGE gel.

Recombinant IDH2 protein, SDS-PAGE gel.
Recombinant IDH2 protein was run on a 12.5% SDS-PAGE gel and stained with Coomassie Blue.
MW: 51.7 kDa
Purity: > 95%

Recombinant IDH2 protein activity.

Recombinant IDH2 protein activity.
200 µM DL-isocitric acid trisodium and 200 µM NADP+ were incubated with IDH2 protein in reaction buffer including 50 mM Tris-HCl pH 7.4, 100 mM MgCl2 for 10 minutes at room temperature. The generation of product NADPH was monitored by measuring absorbance at 340 nm.

Recombinant IDH2 protein activity.

Recombinant IDH2 protein activity.
200 µM DL-isocitric acid trisodium and 200 µM NADP+ were incubated with IDH2 protein in reaction buffer including 50 mM Tris-HCl pH 7.4, 100 mM MgCl2 for 10 minutes at room temperature. The generation of product NADPH was monitored by measuring absorbance at 340 nm.

for Recombinant IDH2 proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant IDH2 proteinGuarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.

 

Technical Data Sheet

Recombinant IDH2 protein
 

Data Thumbnails

Recombinant IDH2 protein, SDS-PAGE gel.

Recombinant IDH2 protein, SDS-PAGE gel.
(Click to enlarge and view details)

Recombinant IDH2 protein activity.

Recombinant IDH2 protein activity.
(Click to enlarge and view details).

Recombinant IDH2 protein activity.

Recombinant IDH2 protein activity.
(Click to enlarge and view details).