COS-7 nuclear extract

for COS-7 nuclear extractContents

2 x 100 µg of COS-7 nuclear extract at 2.5 µg/µl.

for COS-7 nuclear extractBackground

COS-7 nuclear extract was prepared from subconfluent undifferentiated cell cultures of the Simian kidney fibroblast COS-7 cell line. The COS-7 cell line was derived from immortalizing the African green monkey CV-1 cell line by transformation with an origin-defective mutant of the SV40 viral genome that is replication-deficient but produces wild-type large T antigen. The large T antigen supports replication and lytic growth of the SV40 virus as well as replication of SV40 promoter-containing plasmids. In addition to supporting propagation of the Simian SV40 virus, the COS-7 cell line is most commonly used in research related to replication and in transfection studies for the production of recombinant proteins.

for COS-7 nuclear extractApplication Notes

COS-7 nuclear extract is specifically recommended for analysis of replication and for transfection studies.

for COS-7 nuclear extractExtract Origin

Monkey Kidney, SV40 transformed

for COS-7 nuclear extractExtract Composition

COS-7 nuclear extract is supplied in lysis buffer (20 mM Hepes (pH 7.5), 400 mM NaCl, 20% glycerol, 0.1 mM EDTA, 10 mM NaF, 10 µM Na₂MoO₄, 1 mM NaVO₃, 10 mM PNPP, 10 mM beta-glycerophosphate, 1 mM DTT and protease inhibitors).

for COS-7 nuclear extractQuality Control

Extracts have been quality control tested by Western blot.

for COS-7 nuclear extractStorage

To ensure stability, extracts should be stored at -80°C.

We recommend aliquoting the extracts into single-use fractions and then storing them at -80°C. This eliminates repeated freeze/thaw cycles.

for COS-7 nuclear extractGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.