EGF Receptor phospho Tyr992 antibody (pAb)

for EGF Receptor phospho Tyr992 antibody (pAb)Background

EGFR – Epidermal Growth Factor Receptor is a member of the superfamily of transmembrane receptor tyrosine kinases and is widely expressed. Upon ligand binding, EGFR dimerizes, autophosphorylates and becomes active. Many signaling pathways are initiated through the EGFR and related ligands. Phosphorylation of Tyr 992 results in the binding and activation of Phospholipase-C gamma (PLC-gamma).

for EGF Receptor phospho Tyr992 antibody (pAb)Immunogen

This antibody was raised against a synthetic phosphopeptide corresponding to the amino acid residues region that contains Tyr992 of human Phosphorylated-EGFR.

for EGF Receptor phospho Tyr992 antibody (pAb)Application Notes

This EGF Receptor phospho Tyr992 antibody has been validated for use in Western blotting (1:500 - 1:1,000 dilution). For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

for EGF Receptor phospho Tyr992 antibody (pAb)Buffer

12 mM sodium borate, 50 mM boric acid, 37 mM NaCl, 5 mM EDTA and 50% glycerol.

for EGF Receptor phospho Tyr992 antibody (pAb)Positive Control

Active Motif's A-431 nuclear extract (EGF treated) (Catalog No. 36003) can be used as a positive control for EGF Receptor phospho Tyr992 antibody.

for EGF Receptor phospho Tyr992 antibody (pAb)Storage

Antibodies in solution can be stored at -80°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for EGF Receptor phospho Tyr992 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.