| Isotype: | Rabbit IgG |
| Application: | Western blot |
| Reactivity: | Human, Other (Wide Range) |
|
| Purification: | None |
| Molecular Weight: | 14 and 8 kDa |
|
Background:
Histones H2A and H4 are core components of the nucleosome, the basic building block of chromatin. The first five amino acids of histone H2A and histone H4 are identical in metazoans and include an amino-terminal serine residue. Histone H2A phosphorylation at serine 1 is enriched during mitosis, while H4 phosphorylation increases subsequent to DNA double strand breaks.
Immunogen:
This antibody was raised against a peptide including phospho-serine 1 of human histone H2A.
Buffer:
Rabbit serum containing 30% glycerol and 0.035% sodium azide.
Application Notes:
This product has been tested by Western blotting of cells arrested in mitosis (1:1,000 - 1:50,000) and specificity for phospho-serine 1 confirmed by dot blot and peptide inhibition with non-modified peptide or a phospho-Ser1 H2A/H4 peptide. H2A and H4 have divergent amino-terminal sequences in both budding and fission yeasts and thus only detection of histone H4 Ser1 phosphorylation is detected with this antibody.
Acid extract of HeLa cells (5 µg per lane) probed with Catalog No. 39115 (1:30,000 dilution).
Lane 1: no treatment.
Lane 2: cells treated with colcemid.
Storage:
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Guarantee:
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key:
- ChIP = Chromatin Immunoprecipitation;
- FACS = Flow Cytometry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
YOUR CART
View Data Sheet
