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| Western Blot | Chromatin IP |
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| 1:1,000 - 1:5,000 | Not tested |
| View Technical Data Sheet (TDS) |
Background for Histone H2A/H4 phospho Ser1 antibody (pAb):
Histones H2A and H4 are core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.The first five amino acids of histone H2A and histone H4 are identical in metazoans and include an amino-terminal serine residue. Histone H2A phosphorylation at serine 1 is enriched during mitosis, while H4 phosphorylation increases subsequent to DNA double-strand breaks.
Immunogen for Histone H2A/H4 phospho Ser1 antibody (pAb):
This antibody was raised against a peptide including phospho-serine 1 of human histone H2A.Application Notes for Histone H2A/H4 phospho Ser1 antibody (pAb):
This Histone H2A/H4 phospho Ser1 antibody (H2AH4S1Ph) has been validated for use in Western blotting of cells arrested in mitosis (1:1,000 - 1:50,000 dilution); its specificity for phospho-serine 1 was confirmed by dot blot and peptide inhibition with non-modified peptide or a phospho-Ser1 H2A/H4 peptide. Because H2A and H4 have divergent amino-terminal sequences in both budding and fission yeasts, only histone H4 Ser1 phosphorylation is detected with this antibody.Buffer:
Rabbit serum containing 30% glycerol and 0.035% sodium azide.Positive Control:
Active Motif's HeLa acid extract (Paclitaxel treated) (Catalog No. 36201) can be used as a positive control for Histone H2A/H4 phospho Ser1 antibody.Compatible Secondary Antibodies:
Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for your experiments.
Histone H2A/H4 phospho Ser1 pAb tested by Western blot.
HeLa acid extract (5 µg per lane) was probed with Catalog No. 39115 (1:30,000 dilution).
Lane 1: No treatment.
Lane 2: Cells treated with colcemid.
Histone H2A/H4 phospho Ser1 pAb tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H2A/H4 phospho Ser1 pAb for phospho Ser1 of Histone H2A/H4. Modified and unmodified peptides were spotted onto PVDF and probed with the antibody at a 1:10,000 dilution. The amount of peptide spotted (in picomoles) is indicated next to each row.
Lane 1: Peptide phosphorylated at Ser1.
Lane 2: Unmodified Ser1 peptide.
Storage:
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.Guarantee:
This product is guaranteed for 6 months from date of receipt.This product is for research use only and is not for use in diagnostic procedures.
Application Key:
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- IF = Immunofluorescence;
- ICC = Immunocytochemistry;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
