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Histone H2A phospho Ser129 antibody (pAb)
| Catalog No: 39271 | Format: 200 µl | $340 | Buy Now |
| Catalog No: 39272 | Format: 10 µl | $70 | Buy Now |
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| Western Blot | Chromatin IP | Immunofluorescence | Immunoprecipitation |
|---|---|---|---|
| 1:500 - 1:2,000 | 3 - 5 µl per ChIP | 1:250 - 1:1,000 | 3rd-party validated* |
| *This antibody has been reported to work in this application by a third party, but Active Motif has not tested the antibody for this application. Please see "Application Notes" below for any recommendations. | |||
for Histone H2A phospho Ser129 antibody (pAb)Background
Histone H2A is one of the core components of the nucleosome, the basic building block of chromatin. Unlike mammals that have a distinct DNA damage-responsive histone H2A variant, H2AX, budding yeast histone H2A contains an SQ motif in the C-terminus that is phosphorylated by the Mec1 and Tel1 kinases in response to DNA double strand breaks. As with mammalian H2AX phosphorylation, phosphorylation of yeast histone H2A at serine 129 is required for the efficient repair of DNA double-strand breaks.
for Histone H2A phospho Ser129 antibody (pAb)Immunogen
This antibody was raised against a peptide including phospho-serine 129 of budding yeast Histone H2A.
for Histone H2A phospho Ser129 antibody (pAb)Application Notes
This Histone H2A phospho Ser129 antibody (H2AS129Ph) has been validated for use in chromatin IP (3 - 5 µl per ChIP), Western blotting (1:500 - 1:2,000 dilution) and immunofluorescence (1:250 - 1:1,000 dilution), and reported to work in immunoprecipitation.
for Histone H2A phospho Ser129 antibody (pAb)Buffer
Rabbit serum containing 30% glycerol and 0.035% sodium azide.
Chromatin IP Kits & Reagents
Active Motif has a variety of Chromatin Immunoprecipitation kits and reagents for every need. Please visit our Chromatin IP Page to learn about our ChIP kits, controls, chromatin and PCR primers for use with Histone H2A phospho Ser129 antibody (pAb).
Compatible Secondary Antibodies
Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Histone H2A phospho Ser129 antibody (pAb).
Histone H2A phospho Ser129 pAb tested by Western blot.
Whole-cell extract of yeast exposed to 200Gy ionizing radiation blotted with Histone H2A phospho Ser129 pAb at a dilution of 1:1,000.
Lane 1: Yeast containing an alanine at position 129.
Lane 2: Wild-type yeast.
Histone H2A phospho Ser129 pAb tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H2A phospho Ser129 pAb for phospho Ser129 histone H2A. Peptides corresponding to the immunogen and the unmodified version of the immunogen were spotted onto PVDF and probed with the antibody at 1:500. The amount of peptide (picomoles) spotted is indicated next to each row.
Lane 1: Phospho-Ser129 peptide.
Lane 2: Unmodified Ser129 peptide.
for Histone H2A phospho Ser129 antibody (pAb)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for Histone H2A phospho Ser129 antibody (pAb)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
