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Myc Tag antibody (mAb)
| Catalog No: 39279 | Format: 200 µg | $340 | Buy Now |
| Catalog No: 39280 | Format: 10 µg | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP |
|---|---|
| 0.5 - 2 µg/ml | — |
for Myc Tag antibody (mAb)Background
Myc Tag is a sequence derived from the human c-Myc protein that has been developed into an effective epitope for detection of tagged proteins expressed in vivo. Clone 4E12 exhibits superior detection of Myc-tagged proteins compared to the more common 9E10 clone.
for Myc Tag antibody (mAb)Immunogen
This antibody was raised against peptide corresponding to amino acids 410-419 of human c-Myc (EQKLISEEDL).
for Myc Tag antibody (mAb)Application Notes
This Myc Tag antibody has been validated for use in Western blotting (0.5 - 2 µg/ml dilution). Inclusion of 0.05% Tween 20 in the blocking and primary antibody incubation buffers is required for effective detection of Myc Tag fusion proteins by Western Blot.
Clone 4E12 may exhibit preference for Myc tag constructs dependent on the surrounding sequence context.
for Myc Tag antibody (mAb)Buffer
Purified rabbit IgG in PBS with 30% glycerol and 0.035% sodium azide.
for Myc Tag antibody (mAb)Positive Control
Active Motif's NIH/3T3 whole-cell extract (Catalog No. 36418) can be used as a positive control for Myc Tag antibody.
Myc Tag mAb tested by Western blot.
Whole-cell extract (20 µg) of NIH/3T3 cells expressing a Myc-tagged PP2A-alpha subunit blotted with Myc Tag mAb (0.5 µg/ml).
for Myc Tag antibody (mAb)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for Myc Tag antibody (mAb)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
