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Antibodies

Ikaros antibody (mAb)

Clone: 2A9
Aliases: IKZF1, LyF-1, hlk-1, Ikaros, Zfpn1a1, Znfn1a1
Catalog No: 39355 Format: 200 µg $420 Buy Now
Catalog No: 39356 Format: 10 µg $95 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG
Purification:Protein G Chromatography
Host:Mouse
Molecular Weight:62 kDa
Reactivity:Mouse

Applications

ChIP Validated ChIP-Seq Validated Western Blot Validated

for Ikaros antibody (mAb) (Clone 2A9)Application Notes


Validated Applications:
ChIP: 4 µg per ChIP
ChIP-Seq: 4 µg each
WB: 0.5 - 2 µg/ml dilution

ChIP-Seq validation was performed by Active Motif's Epigenetics Services and the complete data set is available in the UCSC Genome Browser by clicking here.

Published Applications: see references

for Ikaros antibody (mAb) (Clone 2A9)Immunogen

This Ikaros antibody was raised against a recombinant protein corresponding to the N-terminal half of mouse Ikaros.

for Ikaros antibody (mAb) (Clone 2A9)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for Ikaros antibody (mAb) (Clone 2A9)References

 
Ikaros antibody (mAb) tested by ChIP-Seq.

Ikaros antibody (mAb) (Clone 2A9) tested by ChIP-Seq.
ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 30 ug of chromatin from mouse spleen and 4 µg of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 15 million sequence tags were mapped to identify Ikaros binding sites. The image shows binding across a region of chromosome 9. You can view the complete data set in the UCSC Genome Browser, starting at this specific location, here.

Ikaros antibody (mAb) tested by Western blot.

Ikaros antibody (mAb) (Clone 2A9) tested by Western blot.
Detection of Ikaros by Western blot analysis. Ikaros detected in 20 µg mouse spleen whole-cell extract using Ikaros mAb (Clone 2A9) at a 2 µg/ml dilution.

for Ikaros antibody (mAb) (Clone 2A9)Background

Ikaros is a transcription factor that plays a critical role in the control of lymphohematopoiesis and immune regulation. All Ikaros isoforms share a common C-terminal domain that contains a transcription activation motif and two zinc finger motifs required for hetero- and homo-dimerization among the Ikaros isoforms, and for interactions with other proteins. Ikaros is abundantly expressed in pituitary mammosomatotrophs, where it deacetylates Histone H3 sites on the proximal growth hormone (GH) promoter to silence gene expression.

for Ikaros antibody (mAb) (Clone 2A9)Positive Control

Whole-cell extracts from mouse spleen can be used as a positive control for Ikaros antibody (Clone 2A9).

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Ikaros antibody (mAb).

Bridging Antibody to Improve ChIP, MeDIP and IP

Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use Ikaros antibody (mAb).

for Ikaros antibody (mAb) (Clone 2A9)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Ikaros antibody (mAb) (Clone 2A9)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

Ikaros antibody (mAb)
 

Data Thumbnails

Ikaros antibody (mAb) tested by ChIP-Seq.

ChIP-Seq of Ikaros mAb.
(Click image to enlarge and see details.)

Ikaros antibody (mAb) tested by Western blot.

Western blot of Ikaros mAb.
(Click image to enlarge and see details.)