Histone H3 di/trimethyl Lys27 antibody (mAb)

for Histone H3 di/trimethyl Lys27 antibody (mAb)Background

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.

The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue. Lysine 27 of histone H3 can be mono-, di- or trimethylated (Histone H3 monomethyl Lys27, Histone H3 dimethyl Lys27 or H3K27me3) by different histone methyltransferases such as EZH2 or NSD3. Methylation of this residue is mainly associated with transcriptional repression.

for Histone H3 di/trimethyl Lys27 antibody (mAb)Immunogen

This Histone H3 di/trimethyl Lys27 antibody was raised against a peptide including trimethyl-lysine 27 of histone H3.

for Histone H3 di/trimethyl Lys27 antibody (mAb)Application Notes

This Histone H3 di/trimethyl Lys27 antibody (H3K27me2/3) has been validated for use in Western blotting (1:500 - 1:2,000 dilution) and chromatin IP (1 - 3 µl per ChIP); specificity for dimethyl and trimethyl lysine 27 was confirmed by peptide competition using peptides methylated at lysines 9 and 27 of H3. Because this modification is conserved in higher eukaryotes, a wide range of reactivity is expected.

This antibody has not been validated in ChIP, but ChIP-validated antibodies are available for this target: 39535.

for Histone H3 di/trimethyl Lys27 antibody (mAb)Buffer

10 mM sodium phosphate pH 7.5, 150 mM NaCl, 30% glycerol, 0.035% sodium azide.

for Histone H3 di/trimethyl Lys27 antibody (mAb)Positive Control

Active Motif's HeLa acid extract (Catalog No. 36200) can be used as a positive control for Histone H3 di/trimethyl Lys27 antibody.

for Histone H3 di/trimethyl Lys27 antibody (mAb)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Histone H3 di/trimethyl Lys27 antibody (mAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.