Histone H4 dimethyl Lys20 antibody (mAb)

for Histone H4 dimethyl Lys20 antibody (mAb)Background

Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.

Histone 4 lysine 20 (H4K20) can be mono-, di- or trimethylated by different histone methyltransferases such as NSD1 or ASH1. The methylation of this lysine is often associated with transcriptional repression.

for Histone H4 dimethyl Lys20 antibody (mAb)Immunogen

This antibody was raised against a peptide including dimethyl-Lys20 of human histone H4.

for Histone H4 dimethyl Lys20 antibody (mAb)Application Notes

This Histone H4 dimethyl Lys20 antibody (H4K20me2) has been validated for use in Western blotting (1:500 - 1:2,000 dilution).

This antibody has not been validated in ChIP, but ChIP-validated antibodies are available for this target: 39173.

for Histone H4 dimethyl Lys20 antibody (mAb)Buffer

Concentrated culture supernatant containing 30% glycerol and 0.035% sodium azide.

for Histone H4 dimethyl Lys20 antibody (mAb)Positive Control

Active Motif's HeLa acid extract (Catalog No. 36200) can be used as a positive control for Histone H4 dimethyl Lys20 antibody.

for Histone H4 dimethyl Lys20 antibody (mAb)References

This antibody was used in Nishioka et al (2002) Molec Cell 9:1201.

for Histone H4 dimethyl Lys20 antibody (mAb)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Histone H4 dimethyl Lys20 antibody (mAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.