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Antibodies

Histone H4K20me2 antibody (mAb)

Catalog No: 39539 Format: 100 µl $410 Buy Now
Catalog No: 39540 Format: 10 µl $95 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG
Purification:Culture Supernatant
Host:Mouse
Molecular Weight:8 kDa
Reactivity:Human, Other (Wide Range)

Applications

Western Blot Validated

for Histone H4 dimethyl Lys20 antibody (mAb)Application Notes


Validated Applications:
WB: 1:500 - 1:2,000 dilution

Published Applications: see references

for Histone H4 dimethyl Lys20 antibody (mAb)Immunogen

This Histone H4 dimethyl Lys20 antibody was raised against a peptide including dimethyl-Lys20 of human histone H4.

for Histone H4 dimethyl Lys20 antibody (mAb)Buffer

Concentrated culture supernatant containing 30% glycerol and 0.035% sodium azide.

for Histone H4 dimethyl Lys20 antibody (mAb)References

 
Histone H4K20me2 antibody (mAb) tested by Western blot.

Histone H4 dimethyl Lys20 mAb tested by Western blot.
Detection of dimethylated Histone H4 by Western blot. The analysis was performed using HeLa acid extract (10 µg, lane 1) and recombinant histone H4 protein (200 ng, lane 2) probed with Histone H4 dimethyl Lys20 mAb at a 1:2,000 dilution.

for Histone H4 dimethyl Lys20 antibody (mAb)Background

Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.

Histone 4 lysine 20 (H4K20) can be mono-, di- or trimethylated by different histone methyltransferases such as NSD1 or ASH1. The methylation of this lysine is often associated with transcriptional repression.

for Histone H4 dimethyl Lys20 antibody (mAb)Positive Control

Active Motif's HeLa acid extract (Catalog No. 36200) can be used as a positive control for Histone H4 dimethyl Lys20 antibody.

for Histone H4 dimethyl Lys20 antibody (mAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Histone H4 dimethyl Lys20 antibody (mAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

Histone H4K20me2 antibody (mAb)
 

Data Thumbnails

Histone H4K20me2 antibody (mAb) tested by Western blot.

Western blot of Histone H4K20me2 mAb.
(Click image to enlarge and see details.)