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Antibodies

Pht1 / H2AZ antibody (pAb)

Catalog No: 39640 Format: 100 µl $340 Buy Now
Catalog No: 39641 Format: 10 µl $70 Buy Now
Antibody Type:Polyclonal
Host:Rabbit
Isotype:IgG
Purification:Affinity Purified
Applications:WB
Reactivity:Fission Yeast
Molecular Weight:15 kDa

Applications Table

Western Blot Chromatin IP
1:500 - 1:1,000

for Pht1 / H2AZ antibody (pAb)Background

Pht1 is the S. pombe homolog of histone H2AZ and is involved in transcriptional regulation and chromosome architecture. It is also implicated in suppressing the accumulation of anti-sense transcripts through interaction with RNAi machinery and heterochromatin proteins.

for Pht1 / H2AZ antibody (pAb)Immunogen

This Pht1 / H2AZ antibody was raised against a peptide corresponding to the C-terminus of Pht1, the histone H2A variant in S. pombe.

for Pht1 / H2AZ antibody (pAb)Application Notes

This Pht1 / H2AZ antibody has been validated for use in Western blotting (1:500 - 1:1,000 dilution).

for Pht1 / H2AZ antibody (pAb)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide.

for Pht1 / H2AZ antibody (pAb)Positive Control

Whole-cell extracts from S. pombe can be used as a positive control for Pht1 / H2AZ antibody.

 
Pht1 / H2AZ pAb tested by Western blot.

Pht1 / H2AZ pAb tested by Western blot.
S. pombe whole-cell extract probed with Pht1 / H2AZ pAb (1:500 dilution).

for Pht1 / H2AZ antibody (pAb)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Pht1 / H2AZ antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FACS = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • WB = Western Blotting
 

Technical Data Sheet

Pht1 / H2AZ antibody (pAb)
 

Data Thumbnails

Pht1 / H2AZ pAb tested by Western blot.

Western blot of Pht1 / H2AZ pAb.
(Click image to enlarge and see details.)