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Antibodies

Ring1B antibody (mAb)

Catalog No: 39663 Format: 100 µg $340 Buy Now
Catalog No: 39664 Format: 10 µg $70 Buy Now
Antibody Type:Monoclonal
Host:Mouse
Isotype:IgG
Purification:Protein G Chromatography
Applications:ChIP, IF, IP, WB
Reactivity:Human, Monkey, Mouse
Molecular Weight:40 kDa

Applications Table

Western Blot Chromatin IP IF IP
Published.
See Application Notes below.		 Published.
See Application Notes below.		 Published.
See Application Notes below.		 Published.
See Application Notes below.

for Ring1B antibody (mAb)Background

Ring1B (also known as ring finger protein 2, RING2 or RNF2) is an essential component of the Polycomb group (PcG) multiprotein PRC1 complex. The PRC1 complex is important for the transcriptional repression of genes involved in development and cell proliferation, such as the Hox genes. Ring1B also acts as an E3 ubiquitin ligase on histone H2A of the PRC1 complex.

for Ring1B antibody (mAb)Immunogen

This Ring1B antibody was raised against the recombinant full-length mouse protein.

for Ring1B antibody (mAb)Application Notes

This Ring1B antibody has been validated for use in the following applications:
   ChIP: see reference 3
   IF: see references 1 & 2
   IP: see references 1, 2 & 4
   WB: see references 1, 2 & 4

for Ring1B antibody (mAb)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 32 mM glycine, 0.07 mM EDTA, 30% glycerol, and 0.035% sodium azide.

Chromatin IP Assays

Active Motif has a variety of Chromatin Immunoprecipitation Kits for every need.
Please visit our Chromatin IP Page to learn about ChIP products designed for use with Ring1B antibody (mAb).

Bridging Antibody to Improve ChIP, MeDIP and IP

Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use Ring1B antibody (mAb).

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Ring1B antibody (mAb).

for Ring1B antibody (mAb)References

1. Atsuta, T. et. al. (2001) Hybridoma 20: 43-46.
2. Suzuki, M. et. al. (2002) Development 129: 4171-4183.
3. Fujimura, Y. et. al. (2006) Development 133: 2371-2381.
4. Isono, K. et. al. (2005) Mol. Cell. Bio. 25: 6694-6706.

for Ring1B antibody (mAb)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Ring1B antibody (mAb)Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FACS = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • WB = Western Blotting
 

Technical Data Sheet

Ring1B antibody (mAb)