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Antibodies

AID antibody (mAb)

Clone: 328.8b
Aliases: AICDA, ARP2, CDA2, HIGM2, HEL-S-284
Catalog No: 39885 Format: 100 µg $410 Buy Now
Catalog No: 39886 Format: 10 µg $95 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG2a
Purification:Protein G Chromatography
Host:Rat
Molecular Weight:24 kDa
Reactivity:Human, Mouse

Applications

ChIP Validated Western Blot Validated

for AID antibody (mAb) (Clone 328.8b)Application Notes


Validated Applications:
WB: 1 - 3 µg/ml dilution

Published Applications:
ChIP
WB

See references for more information. Individual optimization may be required.

for AID antibody (mAb) (Clone 328.8b)Immunogen

This AID antbody was raised against a peptide corresponding to amino acids 178-198 of human AID.

for AID antibody (mAb) (Clone 328.8b)Buffer

Purified IgG in 50 mM Tris-HCl (pH 7.2), 30% glycerol, and 0.035% sodium azide. Sodium azide is highly toxic.

for AID antibody (mAb) (Clone 328.8b)References

 
AID antibody (mAb) tested by Western blot.

AID antibody (mAb) tested by Western blot.
Murine spleen cells were probed with AID antibody at a dilution of 3 µg/ml.

Lane 1: Unstimulated spleen cells from wild type mice.
Lane 2: Treated (LPS & IL4 for 96 hrs) spleen cells from wild type mice.
Lane 3: Unstimulated spleen cells from AID knock-out mice.
Lane 4: Treated (LPS & IL4 for 96 hrs) spleen cells from AID knock-out mice.

for AID antibody (mAb) (Clone 328.8b)Background

AID (Activation-induced cytidine deaminase, AICDA) is a member of the cytidine and deoxycytidylate deaminase family. It acts act as a single-strand DNA deaminase involved in somatic hypermutation, gene conversion and class-switch recombination in developing B-cells and is necessary for efficient antibody responses. It has also been implicated in active DNA demethylation, as deamination of methylcytosine yields thymine, which is read as a mismatch and is replaced by DNA damage repair enzymes.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with AID antibody (mAb).

for AID antibody (mAb) (Clone 328.8b)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for AID antibody (mAb) (Clone 328.8b)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

AID antibody (mAb)
 

Data Thumbnails

AID antibody (mAb) tested by Western blot.

Western blot of AID mAb.
(Click image to enlarge and see details.)