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AID antibody (mAb)
| Clone: 328.8b |
| Catalog No: 39885 | Format: 100 µg | $340 | Buy Now |
| Catalog No: 39886 | Format: 10 µg | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP | IF | IP |
|---|---|---|---|
| 1 - 3 µg/ml | — | 4 µg/ml | 1 - 3 µg per IP |
for AID antibody (mAb) (Clone 328.8b)Background
AID (Activation-induced cytidine deaminase, AICDA) is a member of the cytidine and deoxycytidylate deaminase family. It acts act as a single-strand DNA deaminase involved in somatic hypermutation, gene conversion and class-switch recombination in developing B-cells and is necessary for efficient antibody responses. It has also been implicated in active DNA demethylation, as deamination of methylcytosine yields thymine, which is read as a mismatch and is replaced by DNA damage repair enzymes.
for AID antibody (mAb) (Clone 328.8b)Immunogen
This AID antbody was raised against a peptide corresponding to amino acids 178-198 of human AID.
for AID antibody (mAb) (Clone 328.8b)Application Notes
This AID antibody has been validated for use in immunofluorescence (4 µg/ml), immunoprecipitation (1 - 3 µg per IP) and Western blotting (1 - 3 µg/ml).
for AID antibody (mAb) (Clone 328.8b)Buffer
Purified IgG in 50 mM Tris-HCl (pH 7.2), 30% glycerol, and 0.035% sodium azide.
Compatible Secondary Antibodies
Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with AID antibody (mAb).
AID antibody (mAb) tested by Western blot.
Murine spleen cells were probed with AID antibody at a dilution of 3µg/ml.
Lane 1: Unstimulated spleen cells from wild type mice.
Lane 2: Treated (LPS & IL4 for 96 hrs) spleen cells from wild type mice.
Lane 3: Unstimulated spleen cells from AID knock-out mice.
Lane 4: Treated (LPS & IL4 for 96 hrs) spleen cells from AID knock-out mice.
for AID antibody (mAb) (Clone 328.8b)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for AID antibody (mAb) (Clone 328.8b)Guarantee
This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
