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Antibodies

Histone H2A.Z antibody (pAb)

Catalog No: 39943 Format: 100 µg $420 Buy Now
Catalog No: 39944 Format: 10 µg $95 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Protein A Chromatography
Host:Rabbit
Molecular Weight:14 kDa
Reactivity:Human, Other (Wide Range)

Applications

ChIP Validated ChIP-Seq Validated Western Blot Validated Immunofluorescence Validated Dot Blot Validated Immunocytochemistry Validated

for Histone H2A.Z antibody (pAb) (H2AZ)Application Notes


Validated Applications:
ChIP: 5 - 10 µg per ChIP
ChIP-Seq: 5 - 10 µg each
ICC/IF: 0.5 µg/ml dilution
WB: 0.2 - 1 µg/ml dilution

Published Applications:
WB
See references for more information. Individual optimization may be required.

This histone variant can be perturbed by excessive washing steps prior to fixation. Cells should be washed into serum free culture medium (NOT PBS!) prior to fixation.

for Histone H2A.Z antibody (pAb) (H2AZ)Immunogen

This Histone H2A.Z antibody was raised against a peptide derived from the C-terminus of human histone H2A.Z.

for Histone H2A.Z antibody (pAb) (H2AZ)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. For your convenience, an unpurified serum version (Catalog No. 39113) of this antibody is also available.

for Histone H2A.Z antibody (pAb) (H2AZ)References

 
Histone H2A.Z antibody (pAb) tested by ChIP-Seq.

Histone H2A.Z antibody (pAb) tested by ChIP-Seq.
ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 30 μg of chromatin from a human medulloblastoma cell line and 4 μl of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 18 million sequence tags were mapped to identify H2A.Z occupancy. H2A.Z is found throughout the genome, is often enriched at promoters and depleted from transcribed genes as shown in the images.

Histone H2A.Z antibody (pAb) tested by ChIP.

Histone H2A.Z antibody (pAb) tested by ChIP analysis.
Chromatin IP performed using the ChIP-IT® Express Kit (Catalog No. 53008) and HeLa Chromatin (1.5 x 106 cell equivalents per ChIP) using 10 µg of Histone H2A.Z pAb or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the indicated gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.

Histone H2A.Z antibody (pAb) tested by immunofluorescence.

Histone H2A.Z antibody (pAb) tested by Immunofluorescence.
Formaldehyde fixed HeLa cells stained with Histone H2A.Z antibody at a 0.5 µg/ml dilution.

Histone H2A.Z antibody (pAb) tested by Western blot.

Histone H2A.Z antibody (pAb) tested by Western blot.
Nuclear extract of HeLa cells (25 μg) probed with Histone H2A.Z antibody (pAb) (1 µg/ml). Specificity was verified by peptide competition. Lane 1: no peptide. Lane 2: 1μm immunizing peptide.

Histone H2A.Z antibody (pAb) tested by dot blot analysis.

Histone H2A.Z antibody (pAb) tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H2A.Z antibody for Histone H2A.Z. Recombinant proteins were spotted onto PVDF and probed with Histone H2A.Z antibody at a 1 µg/ml dilution. The amount of peptide spotted (in picomoles) is indicated next to each row.

Lane 1: Recombinant H2A.
Lane 2: Recombinant H2A.Z.
Lane 3: Recombinant H4.

for Histone H2A.Z antibody (pAb) (H2AZ)Background

The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H2A.Z (H2AZ, H2AFZ) is a histone H2A variant, a protein similar to canonical H2A but with different molecular identity and unique functions. H2A.Z is highly conserved during evolution. It plays an important role in basic cellular mechanisms such as gene activation, chromosome segregation, heterochromatic silencing and progression through the cell cycle. H2A.Z is acetylated at multiple lysine residues in its amino terminus, which may serve to allow H2A.Z to function as an insulator of chromatin domains.

for Histone H2A.Z antibody (pAb) (H2AZ)Positive Control

Active Motif's HeLa acid extract (Catalog No. 36200) can be used as a positive control for Histone H2A.Z antibody.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Histone H2A.Z antibody (pAb).

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Histone H2A.Z antibody (pAb).

for Histone H2A.Z antibody (pAb) (H2AZ)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Histone H2A.Z antibody (pAb) (H2AZ)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

Histone H2A.Z antibody (pAb)
 

Data Thumbnails

Histone H2A.Z antibody (pAb) tested by ChIP-Seq.

ChIP-Seq of Histone H2A.Z pAb.
(Click image to enlarge and see details.)

Histone H2A.Z antibody (pAb) tested by ChIP.

ChIP of Histone H2A.Z pAb.
(Click image to enlarge and see details.)

Histone H2A.Z antibody (pAb) tested by immunofluorescence.

Immunofluorescence stain of Histone H2A.Z pAb.
(Click image to enlarge and see details.)

Histone H2A.Z antibody (pAb) tested by Western blot.

Western blot of Histone H2A.Z pAb.
(Click image to enlarge and see details.)

Histone H2A.Z antibody (pAb) tested by dot blot analysis.

Dot blot of Histone H2A.Z pAb.
(Click image to enlarge and see details.)