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Antibodies

Osteoprotegerin (OPG) antibody (mAb)

Clone: 98A1071
Aliases: TNFRSF11B, TR1, OCIF
Catalog No: 40938 Format: 100 µg $410 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG1
Purification:Affinity Purified
Host:Mouse
Molecular Weight:55 kDa
Reactivity:Human

Applications

Western Blot Validated

for Osteoprotegerin (OPG) antibody (mAb) (Clone 98A1071)Application Notes


Validated Applications:
WB: 1 - 2 µg/ml dilution

For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

Published Applications:

WB

for Osteoprotegerin (OPG) antibody (mAb) (Clone 98A1071)Immunogen

This Osteoprotegerin (OPG) antibody was raised against a peptide corresponding to amino acid residues 20-37 of human Osteoprotegerin (OPG).

for Osteoprotegerin (OPG) antibody (mAb) (Clone 98A1071)Buffer

PBS containing 0.02% gelatin and 0.05% sodium azide. Sodium azide is highly toxic.

for Osteoprotegerin (OPG) antibody (mAb) (Clone 98A1071)References

 
Osteoprotegerin (OPG) antibody (mAb) tested by Western blot.

Osteoprotegerin (OPG) mAb tested by Western blot.
Osteoprotegerin (OPG) mAb detection by Western blot. The analysis was performed using Daudi nuclear extract and Osteoprotegerin (OPG) mAb at a 2 µg/ml dilution. The immunoreactivity was detected by enhanced chemiluminescence.

for Osteoprotegerin (OPG) antibody (mAb) (Clone 98A1071)Background

Osteoprotegerin (OPG) / osteoclastogenesis inhibitory factor (OCIF) is a cytokine that can inhibit the production of osteoclasts. It is a member of the tumor necrosis factor (TNF) receptor superfamily. It is a basic glycoprotein comprising 401 amino acid residues arranged into 7 structural domains. It is found as either a 60 kDa monomer or 120 kDa dimer linked by disulfide bonds. Osteoprotegerin inhibits the differentiation of osteoclast precursors into osteoclasts and also regulates the resorption of osteoclasts in vitro and in vivo. Osteoprotegerin is a RANK homolog, and works by binding to RANKL ligand on osteoblast/stromal cells, thus blocking the RANKL-RANK ligand interaction between osteoblast/stromal cells and osteoclast precursors. This has the effect of inhibiting the differentiation of the osteoclast precursor into a mature osteoclast.

for Osteoprotegerin (OPG) antibody (mAb) (Clone 98A1071)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Osteoprotegerin (OPG) antibody (mAb) (Clone 98A1071)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

Osteoprotegerin (OPG) antibody (mAb)
 

Data Thumbnails

Osteoprotegerin (OPG) antibody (mAb) tested by Western blot.

Western blot of Osteoprotegerin (OPG) mAb.
(Click image to enlarge and see details.)