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Antibodies

Histone H2BS14ph antibody (mAb)

Clone: MABI 0251
Catalog No: 61011 Format: 100 µg $410 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG1
Purification:Protein G Chromatography
Host:Mouse
Molecular Weight:15 kDa
Reactivity:Human, Other (Wide Range)

Applications

Western Blot Validated Immunofluorescence Validated Immunocytochemistry Validated

for Histone H2B phospho Ser14 antibody (mAb) (Clone MABI 0251)Application Notes


Published Applications:
ICC/IF
WB
See references for more information. Individual optimization may be required.

for Histone H2B phospho Ser14 antibody (mAb) (Clone MABI 0251)Immunogen

This Histone H2B phospho Ser14 antibody was raised against a peptide containing phospho Ser14 of human histone H2B.

for Histone H2B phospho Ser14 antibody (mAb) (Clone MABI 0251)Buffer

PBS pH 7.5 containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. For your convenience, a PBS only version of this antibody is also available.

for Histone H2B phospho Ser14 antibody (mAb) (Clone MABI 0251)References

for Histone H2B phospho Ser14 antibody (mAb) (Clone MABI 0251)Background

Histone H2B is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins. H2B is phosphorylated at serine 14 in response to DNA damage (dependant upon PI3 kinase kinase activity) and during apoptosis.by the Mst1 kinase.

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Histone H2BS14ph antibody (mAb).

for Histone H2B phospho Ser14 antibody (mAb) (Clone MABI 0251)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Histone H2B phospho Ser14 antibody (mAb) (Clone MABI 0251)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

This antibody is manufactured by MAB Institute, Inc.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

Histone H2BS14ph antibody (mAb)