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Antibodies

YAP1 antibody (pAb)

Aliases: YAP, YKI, YAP2, YAP65
Catalog No: 61293 Format: 100 µl $410 Buy Now
Catalog No: 61294 Format: 10 µl $95 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:75 kDa
Reactivity:Human

Applications

Western Blot Validated Immunoprecipitation Validated

for YAP1 antibody (pAb)Application Notes


Validated Applications:
IP: 10 µl per IP
WB: 1:500 - 1:1,000 dilution

for YAP1 antibody (pAb)Immunogen

This YAP1 antibody was raised against a peptide corresponding to the C-terminal region of human YAP1.

for YAP1 antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for YAP1 antibody (pAb)References

 
YAP1 antibody (pAb) tested by immunoprecipitation.

YAP1 antibody (pAb) tested by Immunoprecipitation.
10 µl of YAP1 antibody was used to immunoprecipitate YAP1 from 400 µg of HeLa whole cell extract (lane 2). 10 µl of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the YAP1 antibody at a dilution of 1:500.

YAP1 antibody (pAb) tested by Western blot.

YAP1 pAb tested by Western blot.
YAP1 detection by Western blot. The analysis was performed using 40 µg HeLa whole-cell extract and YAP1 at a 1:500 dilution.

for YAP1 antibody (pAb)Background

YAP1 (Yes-associated protein 1, YAP, YKI, YAP2, YAP65) is a transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. Is a critical modulator of epidermal stem cell proliferation. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Plays a key role to control cell proliferation in response to cell contact. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. The presence of TEAD transcription factors are required for it to stimulate gene expression, cell growth, anchorage-independent growth, and epithelial mesenchymal transition (EMT) induction.

for YAP1 antibody (pAb)Positive Control

Active Motif's HeLa whole-cell extract (Catalog No. 40050) can be used as a positive control for YAP1 antibody.

for YAP1 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for YAP1 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

YAP1 antibody (pAb)
 

Data Thumbnails

YAP1 antibody (pAb) tested by immunoprecipitation.

Immunoprecipitation of YAP1 pAb.
(Click image to enlarge and see details.)

YAP1 antibody (pAb) tested by Western blot.

Western blot of YAP1 pAb.
(Click image to enlarge and see details.)