Uhrf1 antibody (mAb)
Clone: 1RC-1C10 |
Aliases: Np95, hNP95, ICBP90, RNF106, hUHRF1, huNp95 |
Catalog No: 61341 | Format: 100 µg | Discontinued |
Applications
Application Notes
This product has been discontinued:
Please refer to our alternative product:
Catalog No. 39625
Applications Validated by Active Motif:
ICC/IF: 0.5 µg/ml dilution
WB*: 1:1000 - 1:2,500 dilution
*Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot. In addition, we recommend the addition of 0.05% Tween 20 to all blocking solutions to reduce background.
Published Applications
The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:
ICC/IF
IHC(P)
WB
View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.
Immunogen
This antibody was raised against a synthetic peptide corresponding to amino acid residue(s) 263-793 of human Uhrf1.
Buffer
Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 32 mM glycine, 0.07 mM EDTA, 30% glycerol, and 0.035% sodium azide.
Background
Uhrf1 (ubiquitin-like, containing PHD and RING finger domains 1) is a transcription and cell cycle regulator. It functions as a putative E3 ubiquitin-protein ligase. Uhrf1 is important for G1/S transition of the cell cycle. It is also believed that Uhrf1 may help recruit the DNA methyltransferase, DNMT1, to hemimethylated DNA and assist in the stabilization of DNMT1 with chromatin.
Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
Guarantee
This product is guaranteed for 12 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- WB = Western Blotting