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Antibodies

p300 antibody (mAb)

Clone: NM11
Aliases: EP300, KAT3B, RSTS2
Catalog No: 61401 Format: 100 µg $420 Buy Now
Catalog No: 61402 Format: 10 µg $95 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG1
Purification:Protein A Chromatography
Host:Mouse
Molecular Weight:300 kDa
Reactivity:Human

Applications

ChIP Validated ChIP-Seq Validated Western Blot Validated Immunoprecipitation Validated Immunofluorescence Validated Immunocytochemistry Validated

for p300 antibody (mAb) (Clone NM11)Application Notes


Validated Applications:
ChIP: 5 µg per ChIP
ChIP-Seq: 5 µg each
ICC/IF: 0.5 µg/ml dilution

Published Applications:
ChIP
IP
ICC/IF
WB
See references for more information. Individual optimization may be required.

for p300 antibody (mAb) (Clone NM11)Immunogen

The antibody was raised against full-length p300 purified from human 293 cells.

for p300 antibody (mAb) (Clone NM11)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide.

for p300 antibody (mAb) (Clone NM11)References

 
p300 antibody (mAb) tested by ChIP-Seq.

p300 antibody (mAb) tested by ChIP-Seq.
ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with chromatin from 4.5 million LNCaP cells and 5 µl of p300 antibody. ChIP DNA was sequenced on the Illumina HiSeq and 15 million sequence tags were mapped to identify p300 binding sites. p300 along with H3K4me1 are markers of active enhancer elements and are therefore expected to co-localize. A sampling of the p300 ChIP-Seq data shows the expected co-localization of p300 and H3K4me1.

p300 antibody (mAb) tested by ChIP.

p300 antibody (mAb) tested by ChIP.
ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with chromatin from 4.5 million LNCaP cells and 4 µg of p300 antibody. ChIP DNA was used in qPCR with the negative control primer pair or gene-specific primer pairs as indicated. Data are presented as Binding Events Detected per 1000 Cells using Active Motif's Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.

p300 antibody (mAb) tested by immunofluorescence.

p300 antibody (mAb) tested by Immunofluorescence.
Formaldehyde fixed HeLa cells stained with p300 antibody at a 0.5 µg/ml dilution.

for p300 antibody (mAb) (Clone NM11)Background

p300 (E1A binding protein p300) functions as a histone acetyltransferase and regulates transcription via chromatin remodeling. Acetylates all four core histones in nucleosomes. Histone acetylation gives an epigenetic tag for transcriptional activation. Mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. Also functions as an acetyltransferase for nonhistone targets. Acetylates 'Lys-131' of ALX1 and acts as its coactivator in the presence of CREBBP. Acetylates SIRT2 and is proposed to indirectly increase the transcriptional activity of TP53 through acetylation and subsequent attenuation of SIRT2 deacetylase function. Acetylates HDAC1 leading to its inactivation and modulation of transcription. Can also mediate transcriptional repression. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein.

for p300 antibody (mAb) (Clone NM11)Positive Control

Active Motif's LNCaP nuclear extract (Clone FGC) (Catalog No. 36051) can be used as a positive control for p300 antibody.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with p300 antibody (mAb).

Bridging Antibody to Improve ChIP, MeDIP and IP

Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use p300 antibody (mAb).

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with p300 antibody (mAb).

for p300 antibody (mAb) (Clone NM11)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for p300 antibody (mAb) (Clone NM11)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

p300 antibody (mAb)
 

Data Thumbnails

p300 antibody (mAb) tested by ChIP-Seq.

ChIP-Seq of p300 mAb.
(Click image to enlarge and see details.)

p300 antibody (mAb) tested by ChIP.

ChIP of p300 mAb.
(Click image to enlarge and see details.)

p300 antibody (mAb) tested by immunofluorescence.

Immunofluorescence stain of p300 mAb.
(Click image to enlarge and see details.)