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Antibodies

PPM1D antibody (pAb)

Aliases: WIP1, PP2C-DELTA
Catalog No: 61591 Format: 100 µl $410 Buy Now
Catalog No: 61592 Format: 10 µl $95 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:70 kDa
Reactivity:Human

Applications

Western Blot Validated

for PPM1D antibody (pAb)Application Notes


Validated Applications:
WB: 1:250 - 1:500 dilution

for PPM1D antibody (pAb)Immunogen

This antibody was raised against a peptide within the C-terminal region of human PPM1D.

for PPM1D antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for PPM1D antibody (pAb)References

 
PPM1D antibody (pAb) tested by Western blot.

PPM1D antibody (pAb) tested by Western blot.
T-47D nuclear extract (40 µg) probed with PPM1D antibody at a dilution of 1:500.

for PPM1D antibody (pAb)Background

PPM1D (protein phosphatase 1D) is required for the relief of p53-dependent checkpoint mediated cell cycle arrest. Binds to and dephosphorylates 'Ser-15' of TP53 and 'Ser-345' of CHEK1 which contributes to the functional inactivation of these proteins.

for PPM1D antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for PPM1D antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

PPM1D antibody (pAb)
 

Data Thumbnails

PPM1D antibody (pAb) tested by Western blot.

Western blot of PPM1D pAb.
(Click image to enlarge and see details.)