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Antibodies

GLIS1 antibody (mAb)

Clone: 7-14
Catalog No: 61801 Format: 100 µg $410 Buy Now
Catalog No: 61802 Format: 10 µg $95 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG1k
Purification:Protein G Chromatography
Host:Rat
Molecular Weight:95 kDa
Reactivity:Human, Mouse

Applications

Western Blot Validated Immunofluorescence Validated

for GLIS1 antibody (mAb) (Clone 7-14)Application Notes

Validated Applications:
WB: 0.5 - 2 µg/ml
IF: 1-2 µg/ml

for GLIS1 antibody (mAb) (Clone 7-14)Immunogen

This antibody was raised against a peptide within the amino-terminal half of human GLIS1 (GLI-similar 1), which shares homology with mouse GLIS1.


for GLIS1 antibody (mAb) (Clone 7-14)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

 
GLIS1 antibody (mAb) tested by Western blot.

GLIS1 antibody (mAb) tested by Western Blot.
20 µg of mouse embryonic stem cell (ES cells) nuclear extract was probed with GLIS1 antibody at 2 µg/ml. The MW of GLIS1 is approximately 95 kDa.

GLIS1 antibody (mAb) tested by immunofluorescence.

GLIS1 antibody (mAb) tested by Immunofluorescence.
Formaldehyde-fixed mouse embryonic stem cells (ES cells). Left panel: DAPI staining. Right Panel: GLIS1 antibody.

for GLIS1 antibody (mAb) (Clone 7-14)Background

GLIS1 (Glis Family Zinc Finger 1) belongs to a GLIS subfamily of Krüppel-like zinc-finger transcription factors characterized to regulate a set of downstream targets essential for cellular functions, including pancreatic development, β-cell maturation and maintenance, and insulin production. GLIS1 is enriched in unfertilized eggs and embryos and it can be used to promote direct reprogramming of somatic cells to induced pluripotent stem cells (iPS cells). GLIS1 may regulate the expression of numerous genes, either positively or negatively, and is implicated in mitral valve development, where dysregulation may lead to mitral valve prolapse (MVP).

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with GLIS1 antibody (mAb).

for GLIS1 antibody (mAb) (Clone 7-14)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for GLIS1 antibody (mAb) (Clone 7-14)Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

GLIS1 antibody (mAb)
 

Data Thumbnails

GLIS1 antibody (mAb) tested by Western blot.

Western blot of GLIS1 mAb.
(Click image to enlarge and see details.)

GLIS1 antibody (mAb) tested by immunofluorescence.

Immunofluorescence stain of GLIS1 mAb.
(Click image to enlarge and see details.)