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Recombinant FAK protein

Catalog No: 81108 Format: 20 µg $405 Buy Now
Catalog No: 81808 Format: 1 mg $3,500 Buy Now
Expressed In: Baculovirus Protein Species: Human

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for Recombinant FAK proteinContents

Recombinant FAK protein is supplied in 25 mM HEPES-NaOH pH 7.5, 300 mM NaCl, 10% glycerol, 0.04% Triton X-100, 0.5 mM TCEP.

for Recombinant FAK proteinBackground

FAK (Focal Adhesion Kinase 1), also known as PTK2 (Protein Tyrosine Kinase 2), is a non-receptor protein-tyrosine kinase that plays an essential role in regulating cell migration, adhesion, spreading, reorganization of the actin cytoskeleton, formation and disassembly of focal adhesions and cell protrusions, cell cycle progression, cell proliferation and apoptosis. It is required for early embryonic development and placenta development, embryonic angiogenesis, normal cardiomyocyte migration and proliferation and normal heart development. FAK regulates axon growth and neuronal cell migration, axon branching and synapse formation, and it is required for normal development of the nervous system. It also plays a role in osteogenesis and differentiation of osteoblasts. FAK can function in integrin signal transduction, but also in signaling downstream of numerous growth factor receptors, G-protein coupled receptors (GPCR), EPHA2, netrin receptors and LDL receptors. It can form multisubunit signaling complexes with SRC and SRC family members upon activation; this leads to the phosphorylation of additional tyrosine residues, creating binding sites for scaffold proteins, effectors and substrates. FAK also can regulate numerous signaling pathways, promote activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascade, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling cascade. It can recruit the ubiquitin ligase MDM2 to P53/TP53 in the nucleus, and thereby regulates P53/TP53 activity, P53/TP53 ubiquitination and proteasomal degradation.

for Recombinant FAK proteinApplication Notes

This protein is suitable for use in binding assays, inhibitor screening, and selectivity profiling.

Kinase Activity Assay Conditions: 1 µM TK substrate was incubated with increasing concentrations of FAK protein and 100 µM ATP in reaction buffer for 1 hour. The detection reagents were added and incubated with the reactions for 1 hr. TK antibody was used to detect the products. All operations and reactions were performed at room temperature, and HTRF KinEASE TK assay was used to detect the enzymatic activity.

for Recombinant FAK proteinProtein Details

Recombinant human FAK protein was expressed in a baculovirus expression system as the full length protein (accession number NP_722560.1) with an N-terminal FLAG tag. The molecular weight of the protein is 120.5 kDa.

 
Recombinant FAK protein gel

Recombinant FAK protein gel
5% SDS-PAGE gel, stained with Coomassie Blue
MW: 120.5 kDa
Purity: >65%

HTRF assay for FAK protein activity

HTRF assay for FAK protein activity
1 µM TK substrate was incubated with increasing concentrations of FAK protein and 100 µM ATP in reaction buffer for 1 hour. The detection reagents were added and incubated with the reactions for 1 hr. TK antibody was used to detect the products. All operations and reactions were performed at room temperature, and HTRF KinEASE TK assay was used to detect the enzymatic activity.

HTRF assay for FAK protein activity

HTRF assay for FAK protein activity
1 µM TK substrate was incubated with increasing concentrations of FAK protein and 100 µM ATP in reaction buffer for 1 hour. The detection reagents were added and incubated with the reactions for 1 hr. TK antibody was used to detect the products. All operations and reactions were performed at room temperature, and HTRF KinEASE TK assay was used to detect the enzymatic activity.

for Recombinant FAK proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant FAK proteinGuarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.

 

Technical Data Sheet

Recombinant FAK protein
 

Data Thumbnails

Recombinant FAK protein gel

Recombinant FAK protein gel
(Click to enlarge and view details)

HTRF assay for FAK protein activity

HTRF assay for FAK protein activity
(Click to enlarge and view details)

HTRF assay for FAK protein activity

HTRF assay for FAK protein activity
(Click to enlarge and view details)