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for Recombinant c-Fos proteinContents
5 µg of Recombinant c-Fos protein and Dilution Buffer AM1 (20 mM Tris-Cl (pH 8), 20% glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA). Protein is supplied at 0.5 µg/µl in Dilution Buffer AM1.
for Recombinant c-Fos proteinApplication Notes
Recombinant c-Fos is suitable for DNA and protein-protein interaction assays. 100 ng is sufficient for DNA-protein and protein-protein interaction studies. The molecular weight of the protein is ~50 kDa. The standard curve for TransAM® AP-1 c-Fos was generated using the range of 40-2.5 ng of protein. NOTE: The presence of Poly [d(I-C)] in buffers may affect protein functionality and should be avoided.
for Recombinant c-Fos proteinProtein Details
Recombinant c-Fos was expressed from full length (accession number NM 003131) with an amino terminal polyhistidine tag in a baculovirus system and purified by an affinity column in combination with FPLC chromatography. The purified recombinant protein is greater than 90% homogeneous and contains no detectable protease, DNase and RNase activity.
TransAM® standard curve generated using Recombinant c-Fos protein.
The standard curve for TransAM® was generated using a range of 40-10 ng of protein and run on the TransAM® AP-1 c-Fos ELISA Kit.
for Recombinant c-Fos proteinGuarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
