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Recombinant Proteins & Enzymes

Recombinant Histone H3S10ph (EPL)

Catalog No: 31272 Format: 25 µg $395 Buy Now
Expressed In: E. coli Protein Species: Human

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Expressed Protein Ligation

Recombinant Histone H3S10ph (EPL) has been generated using the patented Expressed Protein Ligation (EPL) technology. EPL can be used to generate methylated, acetylated or phosphorylated histones. In EPL, the histone globular domain is ligated to a peptide that contains the N-terminal histone tail with the desired site-specific modification. The ligation reaction maintains the native histone bonds, and the resulting recombinant protein more closely mimics natural histones.

for Recombinant Histone H3S10ph (EPL)Contents

25 µg supplied as lyophilized powder. Recombinant histones can be resuspended in water or any suitable buffer. We recommend a starting concentration of 1 mg/ml. To fully solubilize the histone we suggest resuspension in the buffer of choice at room temperature for 20-30 minutes with occasional pipetting. Addition of salt or Tris to the resuspension buffer may enhance histone solubility.

for Recombinant Histone H3S10ph (EPL)Background

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 146 base pairs of DNA wrapped around an octamer of core histone proteins (two each of H2A, H2B, H3 and H4). Histone H1 is a linker protein, present at the interface between the nucleosome core and DNA entry/exit points.

for Recombinant Histone H3S10ph (EPL)Application Notes

Recombinant histones are suitable for use as positive controls in the analysis of histone post-translational modifications, as substrates for histone modification enzymes, or to generate chromatin in vitro.

for Recombinant Histone H3S10ph (EPL)Protein Details

Recombinant Histone H3 phospho Ser10 (H3S10ph) proteins are generated using expressed protein ligation (EPL) technology. Truncated human Histone H3.2 is produced in E. coli and purified using FPLC. The purified protein is subsequently ligated to a N-terminal histone tail peptide containing phosphorylated serine 10 via a native peptide bond. The full-length protein is then repurified prior to lyophilization. Protein concentration was determined using the molar extinction coefficient for Histone H3 and absorbance at 280nm. The recombinant histone is >98% pure by SDS-PAGE and confirmed by high-resolution ESI-TOF mass spectrometry. The molecular weight of the recombinant histone is 15,306 Daltons. Please click on the image below to view the mass spectrum.

ESI-TOF mass spectrometry of Recominant Histone H3S10ph (EPL)

 
Recombinant Histone H3 phospho Ser10 analyzed by SDS-PAGE gel.

Recombinant Histone H3 phospho Ser10 tested by SDS-PAGE gel.
SDS-PAGE analysis of 2 µg Recombinant Histone H3 phospho Ser10 (lane 1) and 1 µg Recombinant Histone H3 phospho Ser10 (lane 2).

for Recombinant Histone H3S10ph (EPL)Storage

Lyophilized proteins can be stored at -20°C or -80°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant Histone H3S10ph (EPL)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Recombinant Histone H3S10ph (EPL)
 

Data Thumbnails

Recombinant Histone H3 phospho Ser10 analyzed by SDS-PAGE gel.

SDS-PAGE gel of Recombinant Histone H3 phospho Ser10.
(Click image to enlarge and see details.)