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Recombinant Proteins & Enzymes

Recombinant Histone H3 pan-acetyl

Catalog No: 31289 Format: 25 µg $395 Buy Now
Expressed In: Synthetic Protein Species: Human

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for Recombinant Histone H3 pan-acetylContents

25 µg supplied as lyophilized powder. Recombinant histones can be resuspended in water or any suitable buffer. We recommend a starting concentration of 1 mg/ml. To fully solubilize the histone we suggest resuspension in the buffer of choice at room temperature for 20-30 minutes with occasional pipetting. Addition of salt or Tris to the resuspension buffer may enhance histone solubility.

for Recombinant Histone H3 pan-acetylBackground

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 146 base pairs of DNA wrapped around an octamer of core histone proteins (two each of H2A, H2B, H3 and H4). Histone H1 is a linker protein, present at the interface between the nucleosome core and DNA entry/exit points.

for Recombinant Histone H3 pan-acetylApplication Notes

Recombinant histones are suitable for use as positive controls in the analysis of histone post-translational modifications, as substrates for histone modification enzymes, or to generate chromatin in vitro.

for Recombinant Histone H3 pan-acetylProtein Details

Recombinant Histone H3 pan-acetyl proteins are synthetic modified histones containing acetylation at K4, K9, K14, K18 and K23. The Recombinant Histone H3 pan-acetyl proteins correspond to the native histone sequence and do not contain any amino acid substitutions or residue analogs. Protein concentration was determined using Bradford assay. The recombinant histone is >95% pure by SDS-PAGE and confirmed by high-resolution ESI-TOF mass spectrometry. The molecular weight of the recombinant histone is 15,483 Daltons.

 
SDS-PAGE gel analysis of Recombinant Histone H3 pan-acetyl protein.

SDS-PAGE gel analysis of Recombinant Histone H3 pan-acetyl protein.

for Recombinant Histone H3 pan-acetylStorage

Lyophilized proteins can be stored at -20°C or -80°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in stora

for Recombinant Histone H3 pan-acetylGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Recombinant Histone H3 pan-acetyl
 

Data Thumbnails

SDS-PAGE gel analysis of Recombinant Histone H3 pan-acetyl protein.