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Recombinant Proteins & Enzymes

Recombinant HDAC4 protein, active

Catalog No: 31350 Format: 10 µg $345 Buy Now

Expressed In:

Sf9 cells

Protein Species:

Human

for Recombinant HDAC4 protein, activeContents

5 µg recombinant HDAC4 expressed in Sf9 cells supplied in a buffer of 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 18 mM glutathione, 10% glycerol.

for Recombinant HDAC4 protein, activeApplication Notes

Recombinant HDAC4 is suitable for use in histone deacetylase (HDAC) assays. A good starting point is 2 to 5 ng of enzyme per assay.

for Recombinant HDAC4 protein, activeProtein Details

HDAC4 is a Class II histone deacetylase with broad substrate specificity. Amino acids 627- 1085 of HDAC4 (accession number NP_006028.2) were expressed with an N-terminal GST tag (MW= 75.2 kDa) in Sf9 cells using a baculovirus expression system.

 
HDAC4 activity assay

HDAC4 activity assay.
Recombinant HDAC4 activity measured using a fluorescent HDAC assay.

HDAC4 Coomassie gel

HDAC4 protein gel.
HDAC4 (5 μg) run on an SDS-PAGE gel and stained with Coomassie blue.

for Recombinant HDAC4 protein, activeStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant HDAC4 protein, activeGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Data Thumbnails

HDAC4 activity assay

HDAC4 activity assay.
(Click image to enlarge and see details).

HDAC4 Coomassie gel

HDAC4 Coomassie stained gel.
(Click image to enlarge and see details).