|
|
for Recombinant HDAC4 protein, activeContents
5 µg recombinant HDAC4 expressed in Sf9 cells supplied in a buffer of 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 18 mM glutathione, 10% glycerol.
for Recombinant HDAC4 protein, activeApplication Notes
Recombinant HDAC4 is suitable for use in histone deacetylase (HDAC) assays. A good starting point is 2 to 5 ng of enzyme per assay.
for Recombinant HDAC4 protein, activeProtein Details
HDAC4 is a Class II histone deacetylase with broad substrate specificity. Amino acids 627- 1085 of HDAC4 (accession number NP_006028.2) were expressed with an N-terminal GST tag (MW= 75.2 kDa) in Sf9 cells using a baculovirus expression system.
HDAC4 activity assay.
Recombinant HDAC4 activity measured using a fluorescent HDAC assay.
HDAC4 protein gel.
HDAC4 (5 μg) run on an SDS-PAGE gel and stained with Coomassie blue.
for Recombinant HDAC4 protein, activeStorage
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for Recombinant HDAC4 protein, activeGuarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.


