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Recombinant Proteins & Enzymes

Recombinant SETD2 (1418-1714) protein

Aliases: HYPB; SETD2; HIF-1; HIP-1; KMT3A; HBP231; HSPC069; p231HBP
Catalog No: 31358 Format: 50 µg $405 Buy Now
Expressed In: E. coli Protein Species: Human

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for Recombinant SETD2 (1418-1714) proteinContents

50 µg recombinant SETD2 (1418-1714) expressed in E. coli and supplied in a buffer of 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 16 mM glutathione, 20% glycerol and 3 mM DTT.

for Recombinant SETD2 (1418-1714) proteinBackground

SETD2 (SET Domain Containing 2) is a Histone methyltransferase that specifically trimethylates Lys-36 of histone H3 (H3K36me3) using dimethylated Lys-36 (H3K36me2) as substrate. Represents the main enzyme generating H3K36me3, a specific tag for epigenetic transcriptional activation. SETD2 plays a role in chromatin structure modulation during elongation by coordinating recruitment of the FACT complex and by interacting with hyperphosphorylated POLR2A. SETD2 acts as a key regulator of DNA mismatch repair in G1 and early S phase by generating H3K36me3, a mark required to recruit MSH6 subunit of the MutS alpha complex: early recruitment of the MutS alpha complex to chromatin to be replicated allows a quick identification of mismatch DNA to initiate the mismatch repair reaction. H3K36me3 also plays an essential role in the maintenance of a heterochromatic state, by recruiting DNA methyltransferase DNMT3A. H3K36me3 is also enhanced in intron-containing genes, suggesting that SETD2 recruitment is enhanced by splicing and that splicing is coupled to recruitment of elongating RNA polymerase. Required during angiogenesis. Recruited to the promoters of adenovirus 12 E1A gene in case of infection, possibly leading to regulate its expression.

for Recombinant SETD2 (1418-1714) proteinApplication Notes

Recombinant SETD2 (1418-1714) is suitable for use in histone methyltransferase assays. A good starting point is 50 to 500 ng of enzyme per assay.
Specific Activity: 0.59 pmol/min/μg. This protein is useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Assay conditions: Prepare a 50 μl reaction mix (50 mM TRIS pH 8.8, 5 mM MgCl2, 4 mM DTT, 20 μM S-adenosylmethionine, and varying amounts of SETD2 (1418-1714)) and add to substrate-coated wells. Incubate at room temperature for 1 hr. Add anti-methyl histone H3K36 antibody and incubate 1hr. Next, incubate 1h with the HRP-labeled secondary antibody followed by chemiluminescent detection.

for Recombinant SETD2 (1418-1714) proteinProtein Details

Amino acids 1418-1714 of SETD2 (accession number NM_014159) were expressed in E. coli with an N-terminal GST-Tag (MW = 61 kDa).

 
SETD2 (1418-1714) protein Coomassie gel

Recombinant (SETD2 (1418-1714) protein gel.
SETD2 (1418-1714 protein was run on an SDS-PAGE gel and stained with Coomassie blue.

SETD2 (1418-1714) activity assay

SETD2 (1418-1714) activity assay.
Recombinant SETD2 (1418-1714) activity measured using a chemiluminescent methyltransferase assay.

for Recombinant SETD2 (1418-1714) proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant SETD2 (1418-1714) proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Recombinant SETD2 (1418-1714) protein
 

Data Thumbnails

SETD2 (1418-1714) protein Coomassie gel

SETD2 (1418-1714) protein Coomassie gel.
(Click image to enlarge and see details).

SETD2 (1418-1714) activity assay

SETD2 (1418-1714) activity assay.
(Click image to enlarge and see details).