Active Motif,
Tools to analyze nuclear function,
Your CartYour Cart 0 items
Recombinant Proteins & Enzymes

Recombinant SETD2 (1392-2564) protein

Aliases: HYPB; SET2; HIF-1; HIP-1; KMT3A; HBP231; HSPC069; p231HBP
Catalog No: 31399 Format: 20 µg $425 Buy Now
Expressed In: Baculovirus Protein Species: Human

Request a quote for a bulk orderBulk Order Button


for Recombinant SETD2 (1392-2564) proteinContents

Recombinant SETD2 (1392-2564) protein is supplied in 25 mM HEPES- NaOH pH 7.5, 300 mM NaCl, 5% glycerol, 0.04% Triton X-100, 0.2 mM TCEP. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant SETD2 (1392-2564) proteinBackground

SETD2 (SET Domain Containing 2) is a histone methyltransferase that methylates histone H3 at lysine 36. H3K36 methylation is associated with active chromatin. SETD2 also contains a novel transcriptional activation domain and has been found associated with hyperphosphorylated RNA pol II. Recombinant SETD2 (1392-2564) includes amino acids 1392-2564 that contains the SET domain of SETD2 that catalyzes methylation of lysine residues.

for Recombinant SETD2 (1392-2564) proteinApplication Notes

Recombinant SETD2 (1392-2564) is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.
Specific Activity: H3K36 methyltransferase.
HMT Assay Conditions: 2 µg recombinant nucleosomes were incubated with SETD2 (1392-2564) in reaction buffer for 3 hr at RT. Western blot was used to detect reaction products (H3K36me2 Antibody, Cat# 39891). SETD2 (1392-2564) alone was used as negative control.

for Recombinant SETD2 (1392-2564) proteinProtein Details

Recombinant SETD2 (1392-2564) (accession number NP_054878.5) was expressed in Sf9 cells and contains an N-terminal FLAG-Tag with an observed molecular weight of 134 kDa. The recombinant protein is >85% pure by SDS-PAGE.

 
Recombinant SETD2 (1392-2564) protein gel.

Recombinant SETD2 (1392-2564) protein gel.
SETD2 (1392-2564) protein was run on an 8% SDS-PAGE gel and stained with Coomassie Blue.
MW: 134 kDa.
Purity: > 70%

HMT Assay for Recombinant SETD2 (1392-2564) protein activity.

HMT Assay for Recombinant SETD2 (1392-2564) protein activity. 2 µg recombinant nucleosomes were incubated with SETD2 (1392-2564) in reaction buffer for 3 hr at RT. Western blot was used to detect reaction products (H3K36me2 Antibody, Cat# 39891). SETD2 (1392-2564) alone was used as negative control.

for Recombinant SETD2 (1392-2564) proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant SETD2 (1392-2564) proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Recombinant SETD2 (1392-2564) protein
 

Data Thumbnails

Recombinant SETD2 (1392-2564) protein gel.

Recombinant SETD2 (1392-2564) protein gel.
(Click image to enlarge and see details).

HMT Assay for Recombinant SETD2 (1392-2564) protein activity.

HMT Assay for Recombinant SETD2 (1392-2564) protein activity.
(Click image to enlarge and see details).