Active Motif,
Tools to analyze nuclear function,
Your CartYour Cart 0 items
Recombinant Proteins & Enzymes

Recombinant DNMT3A protein

Aliases: DNA Methyltransferase 3A, DNMT3A2, TBRS, M.HsaIIIA
Catalog No: 31406 Format: 20 µg $395 Buy Now
Catalog No: 31802 Format: 1 mg $3,800 Buy Now
Expressed In: Baculovirus Protein Species: Human

Request a quote for a bulk orderBulk Order Button


for Recombinant DNMT3A proteinContents

Recombinant DNMT3A protein is supplied in 25 mM Tris pH 8.0, 500 mM NaCl, 5% Glycerol, 0.04% Triton X-100, 1mM DTT and 0.2 mg/ml 3× Flag peptide. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant DNMT3A proteinBackground

DNMT3A – (DNA (Cytosine-5-)-Methyltransferase 3) proteins such as DNMT3A (or DNMT 3A) are involved in DNA methylation, in which a methyl group is added to a cytosine residue on DNA, commonly at the C5 position of a CpG dinucleotide. DNMT3A has been shown to be important in the regulation of specific patterns of DNA methylation. Methylation of mammalian DNA has long been recognized to play a major role in a number of cellular functions such as embryonic development, genetic imprinting, X chromosome inactivation and control of gene expression. DNA methylation is generally associated with transcriptional repression.

Three families of DNMTs have been identified: DNMT1, DNMT2 and DNMT3. The DNMT3 family, containing two active methyltransferases, DNMT3A & DNMT3B, and one DNMT3-Like protein (DNMT3L), establishes the initial CpG methylation pattern de novo and shows the same propensity for methylating unmethylated duplex DNA as for hemi-methylated DNA. DNMT3A and DNMT3B are encoded by two distinct genes and are strongly expressed in embryonic stem cells. DNMT3A is ubiquitous in mammalian cells.

for Recombinant DNMT3A proteinApplication Notes

Recombinant DNMT3A protein is suitable for use in enzyme kinetics, inhibitor screening, and selectivity profiling.

DNMT Activity Assay Conditions: Assay was performed using DNMT Activity / Inhibition Assay, Active Motif Catalog No. 55006, for 1 hour at 37°C.

for Recombinant DNMT3A proteinProtein Details

Recombinant DNMT3A protein includes human DNMT3A amino acids 214-912 (accession number NP_783328.1) was expressed in Sf9 cells and contains an N-terminal 6×His-Tag and FLAG-Tag with a molecular weight of 83.8 kDa. The recombinant protein is >95% pure by SDS-PAGE.

 
DNMT3A protein Coomassie gel

Recombinant DNMT3A protein gel.
Recombinant DNMT3A protein was run on an 8% SDS-PAGE gel and stained with Coomassie blue.

DNMT3A protein DNMT assay

Recombinant DNMT3A protein activity assay
Recombinant DNMT3A protein activity was measured using DNMT Activity / Inhibition Assay, Active Motif Catalog No. 55006, for 1 hour at 37°C

for Recombinant DNMT3A proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant DNMT3A proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Recombinant DNMT3A protein
 

Data Thumbnails

DNMT3A protein Coomassie gel

DNMT3A protein Coomassie gel.
(Click image to enlarge and see details).

DNMT3A protein DNMT assay

DNMT3A protein DNMT assay.
(Click image to enlarge and see details).