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Recombinant Proteins & Enzymes

Recombinant SMYD3 protein

Aliases: KMT3E; ZMYND1; ZNFN3A1; bA74P14.1
Catalog No: 31407 Format: 20 μg $395 Buy Now
Expressed In: Baculovirus Protein Species: Human

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for Recombinant SMYD3 proteinContents

Recombinant SMYD3 protein is supplied in 25 mM HEPES-NaOH pH 7.5, 300 mM NaCl, 5% glycerol, 0.04% Triton X-100, 0.2 mM TCEP. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant SMYD3 proteinBackground

SET and MYND domain-containing protein 3 (SMYD3) is a member of the SMYD family of histone methyltransferases that specifically methylates lysine 4 of histone H3 (H3K4), inducing di- and tri-methylation, but not monomethylation. SMYD3 plays an important role in transcriptional activation as a member of an RNA polymerase complex. It has also been shown to bind DNA containing 5'-CCCTCC-3' or 5'-GAGGGG-3' sequences. SMYD3 expression has been shown to be upregulated in certain cancers.

for Recombinant SMYD3 proteinApplication Notes

Recombinant SMYD3 is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.

HMT Assay Conditions: 30 ng/µl MAP3K2 peptide was incubated with 30 ng/µl recombinant SMYD3 protein in reaction buffer including 50 mM TrisCl, pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP, 9 nCi/µl 3H-SAM for 2 hours at room temperature. Activity was detected by fluorography.

for Recombinant SMYD3 proteinProtein Details

Recombinant SMYD3 (accession number NP_001161212.1) was expressed in Sf9 cells and contains an N-terminal FLAG-Tag with an observed molecular weight of 50.7 kDa. The recombinant protein is >95% pure by SDS-PAGE.

 
SMYD3 Coomassie gel

Recombinant SMYD3 protein gel.
SMYD3 protein was run on an SDS-PAGE gel and stained with Coomassie Blue.

for Recombinant SMYD3 proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant SMYD3 proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Recombinant SMYD3 protein
 

Data Thumbnails

SMYD3 Coomassie gel

SMYD3 Coomassie stained gel.
(Click image to enlarge and see details).