Active Motif,
Tools to analyze nuclear function,
Your CartYour Cart 0 items
Recombinant Proteins & Enzymes

Recombinant SMYD2 protein

Aliases: KMT3C, HSKM-B, ZMYND14
Catalog No: 31497 Format: 20 µg $395 Buy Now
Expressed In: Baculovirus Protein Species: Human

Request a quote for a bulk orderBulk Order Button


for Recombinant SMYD2 proteinContents

Full length recombinant SMYD2 protein expressed in Sf9 cells and is supplied in 25 mM Hepes pH 7.5, 300 mM NaCl, 5% Glycerol, 0.04% Triton X-100, 0.2 mM TCEP. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant SMYD2 proteinBackground

SMYD2 (SET and MYND domain containing protein 2) is a lysine methyltransferase that methylates both histones and non-histone proteins. Specifically, SMYD2 methylates histone H3 Lys4 (H3K4me1) and dimethylates histone H3 Lys36 (H3K36me2). SMYD2 also exhibits methyltransferase activity toward non-histone proteins such as p53 and pRb. SMYD2 monomethylates Lys370 of p53, leading to decreased DNA-binding activity and subsequent inhibition of p53-mediated transcriptional regulation. SMYD2 also monomethylates Lys860 of pRb.

for Recombinant SMYD2 proteinApplication Notes

Recombinant SMYD2 is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.

HMT Assay Conditions: 2 ug Calf Thymus Octamer was incubated with different concentrations of recombinant SMYD2 protein in reaction buffer containing 50 mM TrisCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP, 100 μM 3H-SAM for 3 hours at room temperature. Activity was detected by Fluorography.

for Recombinant SMYD2 proteinProtein Details

Recombinant SMYD2 protein (accession number NP_064582.2) was expressed in Sf9 cells and contains an N-terminal FLAG-Tag with a molecular weight of 50.7 kDa. The recombinant protein is >90% pure by SDS-PAGE.

 
SMYD2 protein Coomassie gel

Recombinant SMYD2 protein gel
SMYD2 protein was run on an 8% SDS-PAGE gel and stained with Coomassie blue.

SMYD2 protein activity assay

Recombinant SMYD2 protein activity assay
2 ug Calf Thymus Octamers was incubated with 0.5 ug and 1 ug SMYD2 protein respectivley in reaction buffer containing radioactive 3H-SAM for 3 hours at room temperature. Activity was detected by fluorography.

*Our result showed that H4 may also be methylated by SMYD2.

for Recombinant SMYD2 proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant SMYD2 proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Recombinant SMYD2 protein
 

Data Thumbnails

SMYD2 protein Coomassie gel

SMYD2 protein Coomassie gel.
(Click image to enlarge and see details).

SMYD2 protein activity assay

SMYD2 protein activity assay.
(Click image to enlarge and see details).