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Recombinant Proteins & Enzymes

Recombinant HDAC1 protein

Aliases: Histone Deacetylase 1, HD1, RPD3, GON-10, RPD3L1
Catalog No: 31504 Format: 50 µg $395 Buy Now
Catalog No: 31908 Format: 1 mg $3,500 Buy Now
Expressed In: Baculovirus Protein Species: Human

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for Recombinant HDAC1 proteinContents

Full length recombinant HDAC1 protein was expressed in Sf9 cells and is supplied in 25 mM Hepes pH 7.5, 300 mM NaCl, 5% Glycerol, 0.04% Triton X-100, 0.2 mM TCEP, 0.2 mg/ml 3× Flag peptide. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant HDAC1 proteinBackground

HDAC1 (Histone Deacetylase 1) is a member of the class I mammalian histone deacetylases (HDACs) involved in regulating chromatin structure during transcription. These enzymes catalyze the removal of acetyl groups from lysine residues of histones and other cellular proteins. Lysine N-ε-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in regulation of gene expression in various cellular functions. It consists of the transfer of an acetyl moiety from an acetyl coenzyme A to the ε-amino group of a lysine residue.

In vivo, acetylation is controlled by the antagonistic activities of histone acetyltransferases (HATs) and histone deacetylases (HDACs). The HDACs are grouped into four classes, on the basis of similarity to yeast counterparts: class I (HDAC1, HDAC2, HDAC3 and HDAC8), class II (HDAC4, HDAC5, HDAC6, HDAC7, HDAC9 and 10), class III (SIRT1-7) and class IV (HDAC11).

HDAC1 and HDAC2 are recruited to Mad-Max complexes, which associate with the Sin3 scaffold protein, and are required for the transcriptional repression of Mad-Max target genes. HDAC1 is also involved in the regulation of p53. HDAC1 is expressed in various tissues. HDAC1, HDAC2 and HDAC3 are also ubiquitously expressed and can deacetylate both H3 and H4 in free histones or nucleosome substrate.

for Recombinant HDAC1 proteinApplication Notes

Recombinant HDAC1 is suitable for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Active Motif offers a variety of protein screening tools including HDAC assay kits.

HDAC Activity Assay Conditions: 3 μM Histone H3K9ac (1-21aa) peptide was incubated with HDAC1 protein in reaction buffer including 25 mM Tris-HCl pH 8.0, 137 mM NaCl, 2.7 mM MgCl2, 1 mM KCl and 0.1 mg/ml BSA for 30 min at 37°C. HTRF assay was used for activity detection.

for Recombinant HDAC1 proteinProtein Details

Recombinant human HDAC1 was expressed in Sf9 cells as the full length protein (accession number NP_004955.2) with a C-terminal FLAG-Tag. The molecular weight of the protein is 56 kDa. The recombinant protein is >65% pure by SDS-PAGE.

 
HDAC1 protein Coomassie gel

Recombinant HDAC1 protein gel.
HDAC1 protein was run on an 8% SDS-PAGE gel and stained with Coomassie blue.

HDAC1 protein activity assay

Recombinant HDAC1 activity assay.
3 μM Histone H3K9ac (1-21aa) peptide was incubated with different concentrations of HDAC1 protein in reaction buffer for 30 min at 37°C, Reaction product was detected by Anti-H3K9me0-Eu antibody. HTRF assay was used for activity detection.

for Recombinant HDAC1 proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant HDAC1 proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Recombinant HDAC1 protein
 

Data Thumbnails

HDAC1 protein Coomassie gel

HDAC1 protein Coomassie gel.
(Click image to enlarge and see details).

HDAC1 protein activity assay

HDAC1 protein activity assay.
(Click image to enlarge and see details).