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Antibodies

STAT1α antibody (pAb)

Aliases: CANDF7, ISGF-3, STAT91
Catalog No: 39059 Format: 100 µl $410 Buy Now

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Antibody Type:Polyclonal
Isotype:Serum
Purification:None
Host:Rabbit
Molecular Weight:91 kDa
Reactivity:Human

Applications

Western Blot Validated

for STAT1a antibody (pAb)Application Notes


Validated Applications:
WB: 1:500 - 1:1,000 dilution

For optimal results, primary antibody incubations should be performed at 37°C. Individual optimization may be required.

for STAT1a antibody (pAb)Immunogen

This STAT1α antibody was raised against a synthetic peptide corresponding to amino acid residues 712-750 of human STAT1α.

for STAT1a antibody (pAb)Buffer

PBS containing 0.2% gelatin, 0.01% thimersol and 0.1% sodium azide. Thimersol and sodium azide are highly toxic.

for STAT1a antibody (pAb)References

 
STAT1α antibody (pAb) tested by Western blot.

STAT1α pAb tested by Western blot.
Detection of STAT1α by Western blot analysis. STAT1α was detected in whole-cell extracts derived from T-47D cells using STAT1α rabbit polyclonal antibody at a 1:500 dilution. Several uncharacterized bands of approximately 78 and 140 kDa may also be revealed under the same conditions.

for STAT1a antibody (pAb)Background

STAT1 is alternatively spliced to give rise to STAT1α and STAT1β, both of which mediate interferon α activity, but only STAT1α mediates interferon-γ activity. STAT1 plays a critical role in various forms of cell death, both apoptotic and non-apoptotic. The mechanisms of action are both by transcriptional activation of target genes, and by direct association with apoptotic regulators, including TRADD, p53, and HDAC (HDAC1, HDAC2, HDAC4, HDAC5, HDAC6 and HDAC11). STAT1 target genes include the pro-inflammatory genes for iNOS, COX, VCAM, and ICAM, making STAT1 an attractive anti-inflammatory target.

for STAT1a antibody (pAb)Positive Control

Active Motif's T-47D nuclear extract (Catalog No. 36027) can be used as a positive control for STAT1α antibody.

for STAT1a antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for STAT1a antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

STAT1α antibody (pAb)
 

Data Thumbnails

STAT1α antibody (pAb) tested by Western blot.

Western blot of STAT1α pAb.
(Click image to enlarge and see details.)