Active Motif,
Tools to analyze nuclear function,
Your CartYour Cart 0 items
Antibodies

STAT1 phospho Ser727 antibody (pAb)

Aliases: CANDF7, ISGF-3, STAT91
Catalog No: 39633 Format: 200 µl $410 Buy Now
Catalog No: 39634 Format: 10 µl $95 Buy Now

Request a quote for a bulk orderBulk Order Button


Antibody Type:Polyclonal
Isotype:Serum
Purification:None
Host:Rabbit
Molecular Weight:92 kDa
Reactivity:Human

Applications

Western Blot Validated Dot Blot Validated

for STAT1 phospho Ser727 antibody (pAb)Application Notes


Validated Applications:
WB: 1:500 - 1:1,000 dilution

for STAT1 phospho Ser727 antibody (pAb)Immunogen

This STAT1 phospho Ser727 antibody was raised against a synthetic peptide containing phospho-serine 727 of human STAT1.

for STAT1 phospho Ser727 antibody (pAb)Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for STAT1 phospho Ser727 antibody (pAb)References

 
STAT1 phospho Ser727 antibody (pAb) tested by Western blot.

STAT1 phospho Ser727 pAb tested by Western blot. Lanes 1&4: Untreated HeLa whole-cell extract (20 µg). Lane 2&5: Whole-cell extract (20 µg) of HeLa cells treated with IFNα (2500U/ml for 30 min.). Lanes 3&6: Whole-cell extract (20 µg) of COS-7 cells treated with IFNγ (1000U/ml for 15 min.). Lanes 1-3 were probed with Cat. No. 39633 at 1:500. Lanes 4-6 probed with Cat. No. 39059 (STAT1 alpha pAb) at 1:1000.

STAT1 phospho Ser727 antibody (pAb) tested by dot blot analysis.

STAT1 phospho Ser727 pAb tested by Dot blot. Dot blot analysis was used to confirm the specificity of 39633 for STAT1 phospho Ser727. Phosphorylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with 39633 at 1:500. The amount of peptide (picomoles) spotted is indicated next to each row. Lane 1: unmodified Ser727 STAT1 peptide. Lane 2: phospho Ser727 STAT1 peptide. Lane 3: unmodified Tyr689 STAT2 peptide. Lane 4: phospho Tyr689 STAT2 peptide. Lane 5: unmodified Ser727 STAT3 peptide. Lane 6: phospho Ser727 STAT3 peptide. Lane 7: unmodified Tyr705 STAT3 peptide. Lane 8: phospho Tyr705 STAT3 peptide. Lane 9: unmodified Ser726 STAT5A/Ser731 STAT5B peptide. Lane 10: phospho Ser726 STAT5A/Ser731 STAT5B peptide. Lane 11: unmodified Tyr694 STAT5A/Tyr699 STAT5B peptide. Lane 12: phospho Tyr694 STAT5A/Tyr699 STAT5B

for STAT1 phospho Ser727 antibody (pAb)Background

STAT1 is a member of the STAT family of transcription factors. STATs undergo tyrosine and serine phosphorylation in response to growth factor or cytokine signaling. This phosphorylation results in dimerization and translocation of STAT proteins to the nucleus. STAT1 is alternatively spliced to give rise to STAT1α and STAT1β, both of which mediate interferon α activity, but only STAT1α mediates interferon-γ activity. STAT1 plays a critical role in various forms of cell death, both apoptotic and non-apoptotic. The mechanisms of action are both by transcriptional activation of target genes, and by direct association with apoptotic regulators, including TRADD, p53, and HDAC (HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, HDAC6 and HDAC11). STAT1 target genes include the pro-inflammatory genes for iNOS, COX, VCAM, and ICAM, making STAT1 an attractive anti-inflammatory target.

for STAT1 phospho Ser727 antibody (pAb)Positive Control

Whole-cell extracts from HeLa cells treated with IFNα (2500 U/ml for 30 minutes) can be used as a positive control for STAT1 phospho Ser727 antibody.

for STAT1 phospho Ser727 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for STAT1 phospho Ser727 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

STAT1 phospho Ser727 antibody (pAb)
 

Data Thumbnails

STAT1 phospho Ser727 antibody (pAb) tested by Western blot.

Western blot of STAT1 phospho Ser727 pAb.
(Click image to enlarge and see details.)

STAT1 phospho Ser727 antibody (pAb) tested by dot blot analysis.

Dot blot of STAT1 phospho Ser727 pAb.
(Click image to enlarge and see details.)